What's new for 'Trypanosomatids' in PubMed

This message contains My NCBI what's new results from the National Center for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM).
Do not reply directly to this message.

Sender's message:

Sent on Thursday, 2009 Jul 30
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
Click here to view complete results in PubMed. (Results may change over time.)
To unsubscribe from these e-mail updates click here.

PubMed Results

Items 1 -2 of 2

1: Parasitol Int. 2009 Jul 25. [Epub ahead of print]

Induction of cellular and humoral responses by autoclaved and heat-killed antigen of Leishmania donovani in experimental visceral leishmaniasis.

Nagill R, Mahajan R, Sharma M, Kaur S.

Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh-160014. India Sukhbir Kaur.

The potential of autoclaved and heat-killed antigen of Leishmania donovani to induce cell-mediated and humoral response has been evaluated in the present study. The vaccines were delivered thrice subcutaneously at an interval of 2 weeks. Two weeks after second booster, BALB/c mice were challenged with 10(7) stationary phase promastigotes of L. donovani. Significant protection was achieved in immunized mice against L. donovani challenge with 69% to 76% and 59% to 64% reduction in parasite load in liver and spleen respectively. Immunization induced significantly higher level of delayed type hypersensitivity (DTH) response in mice immunized with heat-killed antigen followed by autoclaved antigen. The immune response was assessed by quantifying Leishmania-specific antibodies and cytokines production. The antibody response was predominantly of IgG type with increased IgG2a production and lesser amount of IgM. The immunization preferentially stimulates the production IFN-gamma and IL-2 in splenocytes which suggest a Th1 type response with a concomitant down-regulation of IL-10 and IL-4. These results indicate a potential for the heat-killed and autoclaved antigen as a vaccine which could trigger cell-mediated immune response.

PMID: 19638322 [PubMed - as supplied by publisher]

Related articles

• A mixed Th1/Th2 response elicited by a liposomal formulation of Leishmania vaccine instructs Th1 responses and resistance to Leishmania donovani in susceptible BALB/c mice.

  Vaccine. 2004 Mar 12; 22(9-10):1162-71.

  [Vaccine. 2004]

• Immune responses induced by the Leishmania (Leishmania) donovani A2 antigen, but not by the LACK antigen, are protective against experimental Leishmania (Leishmania) amazonensis infection.

  Infect Immun. 2003 Jul; 71(7):3988-94.

  [Infect Immun. 2003]

• Adjuvanticity and protective immunity elicited by Leishmania donovani antigens encapsulated in positively charged liposomes.

  Infect Immun. 1997 Jun; 65(6):2371-7.

  [Infect Immun. 1997]

• Cross-protective efficacy of a prophylactic Leishmania donovani DNA vaccine against visceral and cutaneous murine leishmaniasis.

  Infect Immun. 2005 Feb; 73(2):812-9.

  [Infect Immun. 2005]

• gp63 in stable cationic liposomes confers sustained vaccine immunity to susceptible BALB/c mice infected with Leishmania donovani.

  Infect Immun. 2008 Mar; 76(3):1003-15. Epub 2008 Jan 14.

  [Infect Immun. 2008]

• » See reviews... | » See all...

2: Rev Biol Trop. 2009 Mar-Jun;57(1-2):23-31.

[Asymmetric reproductive isolation between Lutzomyia pseudolongipalpis and Lutzomyia longipalpis (species C2), neotropical vectors of visceral leishmaniasis (Diptera: Pshychodidae)]

[Article in Spanish]

Arrivillaga J, Salerno P, Rangel Y.

Ecología molecular de insectos vectores, Universidad Simón Bolívar, Caracas, Sarteneja, Venezuela. jazzmin.arrivillaga@gmail.com

Lutzomyia pseudolongipalpis and Lutzomyia longipalpis (species C2, L. sp n.) are two endemic species of Phlebotominae sand fly vectors from Venezuela. The two insects are sympatric and monophyletic but have deeply diverging, morphological differences. They belong to the L. longipalpis complex. A study of their reproductive isolation is necessary to understand the process of speciation and maintenance of the two sister species as two discrete taxonomic and biological entities. Cross-mating tests were conducted (homo and hetero-specific) and monitored under two criteria: biological (presence of copulation and offspring) and genetic (using two isozymic markers diagnostic for the L. longipalpis complex; enzyme diagnosis AK and HK). Results indicate reproductive isolation, with an asymmetrical genetic exchange in a direction of hybridization between the two species under experimental conditions, and production of a low number of heterozygotes. These findings support the existence of negative selection on hybrids, and explain the absence of hybrids under natural conditions, in the sympatric locality.

PMID: 19637685 [PubMed - in process]

Related articles

• Lutzomyia pseudolongipalpis: the first new species within the longipalpis (Diptera: Psychodidae: Phlebotominae) complex from La Rinconada, Curarigua, Lara State, Venezuela.

  J Med Entomol. 2001 Nov; 38(6):783-90.

  [J Med Entomol. 2001]

• Activity of Lutzomyia pseudolongipalpis and L. longipalpis s.l. (Diptera: Psychodidae) in Venezuela.

  Parasite. 2004 Sep; 11(3):273-8.

  [Parasite. 2004]

• Occurrence of sibling species of Lutzomyia longipalpis (Diptera: Psychodidae) in Venezuela: first evidence from reproductively isolated sympatric populations.

  Am J Trop Med Hyg. 1999 Dec; 61(6):1004-9.

  [Am J Trop Med Hyg. 1999]

• ReviewHost races in plant-feeding insects and their importance in sympatric speciation.

  Philos Trans R Soc Lond B Biol Sci. 2002 Apr 29; 357(1420):471-92.

  [Philos Trans R Soc Lond B Biol Sci. 2002]

• ReviewVectors of leishmaniases in Venezuela.

  Parassitologia. 1991 Dec; 33 Suppl:229-36.

  [Parassitologia. 1991]

• » See reviews... | » See all...