What's new for 'Trypanosomatids' in PubMed

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Sent on Friday, 2009 Nov 27
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

Items 1 -3 of 3

1.	Vet Parasitol. 2009 Oct 31. [Epub ahead of print] Detection of Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) infantumchagasi in Brazilian bats. Savani ES, de Almeida MF, de Oliveira Camargo MC, D'Auria SR, Silva MM, de Oliveira ML, Sacramento D. Centro de Controle de Zoonoses do Município de São Paulo, Rua Santa Eulália 86, 02031-020 Santana, São Paulo, SP, Brazil. Although bats are one of the most abundant mammals in the new world and are present in virtually all ecosystems, including urban and peri-urban environments, few studies have investigated the role of these animals in the epidemiological chain of leishmaniosis. Here, we report a study of 683 bats captured in São Paulo county (southeastern from Brazil), which were screened by serology, parasitologic methods and polymerase chain reaction (PCR) for trypanosomatids. The indirect immunofluorescent antibody test demonstrated that 0.9% of bats react positively for leishmaniosis and PCR detected the presence of DNA of Leishmania (Leishmania) amazonensis in 18 bats and Leishmania (Leishmania) infantumchagasi in 3 specimens. These results indicate that further studies are necessary to evaluate the role of bats in maintenance of the Leishmania life cycle, especially in areas where these diseases are endemic.
	PMID: 19939568 [PubMed - as supplied by publisher]

2.	Bull Entomol Res. 2009 Nov 26:1-6. [Epub ahead of print] Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Psychodidae): indistinguishable by the mitochondrial cytochrome b gene in Iran. Parvizi P, Taherkhani H, Ready PD. Molecular Systematics Laboratory, Pasteur Institute of Iran, Tehran, Iran. Diagnostic molecular markers for the females of Phlebotomus (Paraphlebotomus) caucasicus and P. mongolensis were sought by characterizing from individual Iranian specimens a gene fragment, namely mitochondrial cytochrome b, that had previously proven useful for the taxonomy of phlebotomine sandflies. Males of both species were used as reference material because their external genitalia provide the only diagnostic morphological characters. A phylogenetic analysis of the new sequences, and those previously reported for P. grimmi, found no support for recognizing more than one species (P. caucasicus s.l.) in Iran. Most of the genetic variation was geographical. An absence of lineage sorting was demonstrated, and it is proposed that any search for species-specific molecular markers for these three taxonomic species should be continued by applied biologists only if there is better evidence for associating any one of them with phenotypes important for understanding the transmission of Leishmania species in foci of zoonotic cutaneous leishmaniasis.
	PMID: 19939318 [PubMed - as supplied by publisher]

3.	BMC Infect Dis. 2009 Nov 25;9(1):186. [Epub ahead of print] Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients. Flechas ID, Cuellar A, Cucunuba ZM, Rosas F, Velasco V, Steindel M, Thomas MD, Lopez MC, Gonzalez JM, Puerta CJ. ABSTRACT: BACKGROUND: Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude T. cruzi extract and three recombinant antigens: the T. cruzi kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the T. cruzi HSP-70 protein192-433, and the entire Trypanosoma rangeli HSP-70 protein. METHODS: Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests. RESULTS: The T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass. CONCLUSIONS: T. cruzi KMP-11 and the T. rangeli HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease. Polarising the IgG1 subclass of the IgG response to T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.
	PMID: 19939275 [PubMed - as supplied by publisher]