This message contains My NCBI what's new results from the National Center for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM).
Do not reply directly to this message.
Sender's message:
Sent on Friday, 2010 Feb 19Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
Click here to view complete results in PubMed. (Results may change over time.)
To unsubscribe from these e-mail updates click here.
| PubMed Results |
| 1. | Parasitol Res. 2010 Feb 18. [Epub ahead of print]Isolation of an antileishmanial and antitrypanosomal flavanone from the leaves of Baccharis retusa DC. (Asteraceae).Grecco SS, Reimão JQ, Tempone AG, Sartorelli P, Romoff P, Ferreira MJ, Fávero OA, Lago JH.Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo-Campus Diadema, 09972-270, Diadema, São Paulo, Brazil. In the course of selection of new bioactive compounds from Brazilian flora, the crude MeOH extract from the leaves of Baccharis retusa DC. (Asteraceae) showed potential against Leishmania sp. and Trypanosoma cruzi. Chromatographic fractionation of the dichloromethane phase from MeOH extract yielded great amounts of the bioactive derivative, which was characterized as 5,6,7-trihydroxy-4'-methoxyflavanone. The structure of this compound was established on the basis of spectroscopic data analysis, mainly nuclear magnetic resonance and mass spectrometry. |
| PMID: 20165875 [PubMed - as supplied by publisher] | |
| Related articles | |
| 2. | J Clin Microbiol. 2010 Feb 17. [Epub ahead of print]Application of an improved ELISA method for the serological diagnosis of canine leishmaniasis.Santarém N, Silvestre R, Cardoso L, Schallig H, Reed SG, Cordeiro-da-Silva A.Parasite Disease Group, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal; Departamento de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Portugal; Departamento de Ciências Veterinárias, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal; KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute), KIT Biomedical Research, Amsterdam, The Netherlands; Infectious Disease Research Institute, Seattle, WA, USA. Accurate diagnosis of canine leishmaniasis (CanL) is essential towards a more efficient control of this zoonosis, but remains problematic due to the high incidence of asymptomatic infections. In this study, data on the development of ELISA-based techniques for the detection of antibodies against the recombinant protein LicTXNPx and compared the results with those employing soluble Leishmania antigens from promastigote or amastigote forms and the homologue recombinant protein LimTXNPx is presented. Moreover, an evaluation of the diagnostic potential of rK39 for CanL in the Portuguese canine population and propose an improvement on the existing ELISA-based serological techniques by combining the LicTXNPx and rK39 antigens, which were designated LAM (Leishmania antigen mixture) is performed. The data demonstrated that ELISAs based on soluble promastigote or amastigote antigens had generally higher sensitivities in symptomatic or asymptomatic dogs when compared to isolated recombinant proteins. Nevertheless, the specificities were found to be similar for all target antigens used. Importantly, the LAM-ELISA methodology improved the overall sensitivity maintaining a high overall specificity. In addition, it was demonstrated that the detection of anti-LAM IgG2 can increase the accuracy of the serological diagnosis. Overall, the obtained results showed that the strategy of combining two well defined Leishmania antigens, LicTXNPx and rK39, proved to be a sensitive and specific improvement of current serological diagnosis of CanL being a useful tool for the detection of both clinical and subclinical forms of the canine Leishmania infection. |
| PMID: 20164286 [PubMed - as supplied by publisher] | |
| Related articles | |
| 3. | Expert Opin Pharmacother. 2010 Mar;11(4):557-69.Cutaneous and mucocutaneous leishmaniasis: emerging therapies and progress in disease management.Ameen M.Royal Free Hospital, Dermatology Department, London, NW3 2QG, UK +00 44 207 7940 500 ; +00 44 207 8302 247 ; mahreenameen@hotmail.com. Importance of the field: Cutaneous leishmaniasis (CL) is a major tropical skin disease. Its incidence continues to increase, and disease control and management are challenging. Available therapies remain inadequate and are associated with low efficacy, toxicity, difficulties with administration, or are expensive. Areas covered in this review: This article describes progress in the therapeutics of CL since 2006. Clinical trials have provided further evidence for the use of alternative systemic agents to first-line antimonials, an enhanced topical paromomycin preparation, the efficacy of thermotherapy, photodynamic therapy as an emerging physical therapy, and the role of immunotherapy and immunomodulators as adjuncts to chemotherapy. In addition, in vitro studies have demonstrated the anti-leishmanial effects of several drugs, which might represent potential future therapeutic agents for CL. What the reader will gain: An overview of the magnitude and complexity of this heterogenous disease, and an update on recent advances in therapeutics and future directions for new drug development. Take home message: Drug therapy for CL must be tailored according to infective species, endemic region, and host responses; a range of different therapies and modalities is therefore required. The impetus for new drug development must continue, combination therapies need to be evaluated, and robust and comparative trials of existing agents are required to adequately assess their efficacy and tolerability. |
| PMID: 20163267 [PubMed - in process] | |
| Related articles | |
| 4. | US Army Med Dep J. 2009 Jul-Sep:28-32.Applications of ecological niche modeling to enhance medical threat assessment and disease control and p revention strategies.Colacicco-Mayhugh M.Department of Sand Fly Biology, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA. |
| PMID: 20084735 [PubMed - indexed for MEDLINE] | |
| Related articles | |
MeSH Terms:
|
| 5. | J Clin Microbiol. 2009 Dec;47(12):3945-51. Epub 2009 Oct 21.Evaluation of adult chronic Chagas' heart disease diagnosis by molecular and serological methods.Ramírez JD, Guhl F, Umezawa ES, Morillo CA, Rosas F, Marin-Neto JA, Restrepo S.Facultad de Ciencias, Departamento de Ciencias Biológicas, Centro de Investigaciones en Microbiología y Parasitología Tropical, CIMPAT, Universidad de los Andes, Carrera 1 No 18A 10, Bogotá, Colombia. Chagas' disease caused by Trypanosoma cruzi is endemic in Latin America. T. cruzi presents heterogeneous populations and comprises two main genetic lineages, named T. cruzi I and T. cruzi II. Diagnosis in the chronic phase is based on conventional serological tests, including indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA), and diagnosis in the acute phase based on parasitological methods, including hemoculture. The objective of this study was to evaluate the diagnostic procedures of Chagas' disease in adult patients in the chronic phase by using a PCR assay and conventional serological tests, including TESA-blot as the gold standard. Samples were obtained from 240 clinical chronic chagasic patients. The sensitivities, compared to that of TESA-blot, were 70% for PCR using the kinetoplast region, 75% for PCR using the nuclear repetitive region, 99% for IIF, and 95% for ELISA. According to the serological tests results, we recommend that researchers assess the reliability and sensitivity of the commercial kit Chagatest ELISA recombinant, version 3.0 (Chagatest Rec v3.0; Wiener Lab, Rosario, Argentina), due to the lack of sensitivity. Based on our analysis, we concluded that PCR cannot be validated as a conventional diagnostic technique for Chagas' disease. These data have been corroborated by low levels of concordance with serology test results. It is recommended that PCR be used only for alternative diagnostic support. Using the nuclear repetitive region of T. cruzi, PCR could also be applicable for monitoring patients receiving etiologic treatment. PMCID: PMC2786654 [Available on 2010/6/1] |
| PMID: 19846646 [PubMed - indexed for MEDLINE] | |
| Related articles | |
 | |
Publication Types:
MeSH Terms:
Substances:
Grant Support:
|
| 6. | Microsc Res Tech. 2010 Jan;73(1):45-50.Structural study of the salivary glands of Anocentor nitens (Acari: Ixodidae) during the feeding cycle.da Silva VC, Pinheiro NL, Ribeiro VR, de Carvalho RW, Scherer PO, Dos Santos MA, Dos Santos-Mallet JR.Reference Laboratory for Entomological Surveillance of Leishmaniasis Vectors, Oswaldo Cruz Institute/FIOCRUZ, Av Brasil 4365, Rio de Janeiro-RJ, Brazil. The salivary glands of Anocentor nitens (Neumann,1897) occur in pairs and are located in the anterolateral region of the general cavity, with milky white color and approximately equal sizes. They consist of a secretory portion and an excretion duct. In some glandular acini, all the cells had a basophilic appearance they were stained by hematoxylin, whereas others presented cells with different staining affinities. In this work, we describe the variations observed in these glands during the feeding cycle of ticks [after feeding (0 h) and successively at 24, 48, 72, 96, 120, and 144 h]. The cells stained by hematoxylin were shown to be more reactive to Alcian blue, thus demonstrating the presence of acid glycosaminoglycans, whereas those stained using eosin presented weak or no reaction. A strong reaction was found by the use of the periodic acid-Schiff (PAS) technique, thereby suggesting the presence of glycogen and/or glycoconjugates containing hexose, confirmed by using salivary amylase before PAS, with partial destaining of the slides. Continuing presence of residual staining in these cells suggests the presence of glycoconjugates containing hexose. Cells with nuclei of circular outline and few granules (of different sizes) were found in type II acini, 72 h after collection. Type I acini presented wide lumina and walls composed of larger numbers of cells of cubic to cylindrical shape. The pronounced degranulation shown in this study over the course of the feeding cycle was associated with the release of substances for oviposition. (c) 2009 Wiley-Liss, Inc. |
| PMID: 19544533 [PubMed - indexed for MEDLINE] | |
| Related articles | |
 | |
MeSH Terms:
|
No comments:
Post a Comment