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Sent on Wednesday, 2010 May 19Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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| PubMed Results |
| 1. | Infect Genet Evol. 2010 May 14. [Epub ahead of print]Targeted gene expression profiling in Leishmania braziliensis and Leishmania guyanensis parasites isolated from Brazilian patients with different antimonial treatment outcomes.Torres DC, Adaui V, Ribeiro-Alves M, Romero GA, Arévalo J, Cupolillo E, Dujardin JC.Laboratório de Pesquisa em Leishmaniose/Coleção de Leishmania do Instituto Oswaldo Cruz IOC-Fiocruz, Rio de Janeiro, Brazil; Laboratório de Biologia Computacional e Sistemas/Instituto Oswaldo Cruz IOC-Fiocruz, Rio de Janeiro, Brazil. AbstractIn Brazil, cutaneous leishmaniasis represents a serious public health problem, and chemotherapy is an important element of the clinical management of this disease. However, treatment efficacy is variable, a phenomenon that might be due to host and parasite (e.g., drug resistance) factors. To better understand the possible contribution of parasite factors to this phenomenon, we characterised 12 Leishmania braziliensis (LB) and 25 L. guyanensis (LG) isolates collected from patients experiencing different antimonial treatment outcomes. For each isolate, promastigote cultures were grown in duplicate and were harvested at the late-log and stationary phases of growth. The RNA expression profiles of six genes encoding proteins with roles in antimony metabolism (AQP1, MRPA, GSH1, GSH2, TRYR and TDR1) were assessed by means of real-time quantitative PCR. Molecular data were compared to the clinical phenotypes. Within LB, we did not find statistically significant differences in the expression levels of the examined genes among isolates from patients with different treatment outcomes. In LG, GSH1 (encoding gamma-glutamylcysteine synthetase, gamma-GCS) was overexpressed in therapeutic failure isolates regardless of the growth curve phase. This finding reveals the predictive potential of promastigote expression curves for the prognosis of cutaneous leishmaniasis caused by LG in Brazil. Copyright © 2010. Published by Elsevier B.V. |
| PMID: 20478409 [PubMed - as supplied by publisher] | |
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| 2. | Infect Genet Evol. 2010 May 14. [Epub ahead of print]Lovesongs and period gene polymorphisms indicate Lutzomyia cruzi (Mangabeira, 1938) as a sibling species of the Lutzomyia longipalpis (Lutz and Neiva, 1912) complex.Vigoder FM, Araki AS, Bauzer LG, Souza NA, Brazil RP, Peixoto AA.Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil. AbstractThe sand fly Lutzomyia cruzi (Mangabeira, 1938) is implicated as a vector of American visceral leishmaniasis (AVL) in some areas of Brazil. Lutzomyia cruzi is closely related to Lutzomyia longipalpis s.l, (Lutz and Neiva, 1912) the main Latin American vector of AVL and a species complex. Although females of the two species are identical, the males can be distinguished by differences in the genitalia. Nevertheless, pheromone analysis shows that Lu. cruzi males produce 9-methyl-germacrene-B, which has also been found in a number of Latin American populations of Lu. longipalpis s.l. In addition, analysis of microsatellite loci shows that the level of divergence between Lu. cruzi and Lu. longipalpiss.l. is similar to that observed among the Lu. longipalpiss.l. sibling species. Here we present the lovesongs of Lu. cruzi males which are similar to the Burst-type songs produced by one of the Lu. longipalpiss.l. sibling species. We also present data on the molecular polymorphisms of the period gene of Lu. cruzi that indicate this species as another sibling within the Lu. longipalpis complex. The results highlight the importance of an integrative approach to understand the patterns of genetic and phenotypic divergence among very closely related vector species. Copyright © 2010. Published by Elsevier B.V. |
| PMID: 20478408 [PubMed - as supplied by publisher] | |
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| 3. | Lipids. 2010 Mar;45(3):275-83. Epub 2010 Feb 4.Fetal bovine serum concentration affects delta9 desaturase activity of Trypanosoma cruzi.Villasuso AL, Romero P, Woelke M, Moyano P, Machado E, de Lema MG.Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, CPX5804BYA, Río Cuarto, Córdoba, Argentina. AbstractFetal bovine serum (FBS) is an important factor in the culture of Trypanosoma cruzi, since this parasite obtains and metabolizes fatty acids (FAs) from the culture medium, and changes in FBS concentration reduce the degree of unsaturation of FAs in phosphoinositides. When T. cruzi epimastigotes were cultured with 5% instead of 10% FBS, and stearic acid was used as the substrate, (9) desaturase activity decreased by 50%. Apparent K (m) and V (m) values for stearic acid, determined from Lineaweaver-Burk plots, were 2 microM and 219 pmol/min/mg of protein, respectively. In studies of the requirement for reduced pyridine nucleotide, (9) desaturase activity reached a maximum with 8 microM NADH and then remained constant; the apparent K (m) and V (m) were 4.3 microM and 46.8 pmol/min/mg of protein, respectively. The effect of FBS was observed only for (9) desaturase activity; (12) desaturase activity was not affected. The results suggest that decreased FBS in culture medium is a signal that modulates (9) desaturase activity in T. cruzi epimastigotes. |
| PMID: 20131019 [PubMed - indexed for MEDLINE] | |
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