Wednesday, June 2, 2010

What's new for 'Trypanosomatids' in PubMed

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Sent on Wednesday, 2010 Jun 02
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results
Items 1 - 6 of 6

1. J Am Vet Med Assoc. 2010 Jun 1;236(11):1221-4.

Comparison of urine protein-to-creatinine ratio in urine samples collected by cystocentesis versus free catch in dogs.

Beatrice L, Nizi F, Callegari D, Paltrinieri S, Zini E, D'Ippolito P, Zatelli A.

Sezione di Clinica Medica Veterinaria, Dipartimento di Salute Animale, University of Parma, 43100 Parma, Italy.

Abstract

Objective-To assess whether urine protein-to-creatinine (UPC) ratios determined in urine samples collected by cystocentesis versus those collected by free catch provide similar diagnostic information for dogs. Design-Evaluation study. Animals-115 client-owned dogs evaluated because of various health problems requiring urinalysis or to screen for proteinuria in an area endemic for leishmaniasis. Procedures-230 paired urine samples, 1 collected by cystocentesis and 1 by free catch, were collected from the 115 dogs. The UPC ratio was determined in paired urine samples (n = 162) from 81 dogs with no indication of active inflammation according to urine sediment analysis. On the basis of the UPC ratio of urine sample collected by cystocentesis, dogs were classified as nonproteinuric (UPC ratio < 0.2), borderline proteinuric (UPC ratio of 0.2 to 0.5), or proteinuric (UPC ratio > 0.5), according to the International Renal Interest Society (IRIS). Results-The correlation between UPC ratio in urine samples collected by cystocentesis and by free catch was strong (r(2) = 0.90); 75 of 81 (92.6%) dogs had UPC ratios from both urine samples that resulted in classification in the same IRIS substage with a kappa coefficient of 0.83. Conclusions and Clinical Relevance-The UPC ratio in dogs was minimally affected in urine samples collected by free catch, thus allowing correct grading of proteinuria with this method. The high reliability of the UPC ratio in free-catch urine samples coupled with the ease of collection should increase the use of this value for assessment of proteinuria.

PMID: 20513201 [PubMed - in process]
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2. J Am Vet Med Assoc. 2010 Jun 1;236(11):1200-6.

Guidelines for prevention of leishmaniasis in dogs.

Maroli M, Gradoni L, Oliva G, Castagnaro M, Crotti A, Lubas G, Paltrinieri S, Roura X, Zini E, Zatelli A.

Vector-borne Diseases & International Health Unit, MIPI Department, National Institute of Health, Viale Regina Elena, 00161 Rome, Italy.

PMID: 20513198 [PubMed - in process]
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3. J Am Vet Med Assoc. 2010 Jun 1;236(11):1192-8.

Guidelines for treatment of leishmaniasis in dogs.

Oliva G, Dvm XR, Crotti A, Maroli M, Castagnaro M, Gradoni L, Lubas G, Paltrinieri S, Zatelli A, Zini E.

Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Naples, 80137 Naples, Italy.

PMID: 20513197 [PubMed - in process]
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4. J Am Vet Med Assoc. 2010 Jun 1;236(11):1184-91.

Guidelines for diagnosis and clinical classification of leishmaniasis in dogs.

Paltrinieri S, Solano-Gallego L, Fondati A, Lubas G, Gradoni L, Castagnaro M, Crotti A, Maroli M, Oliva G, Roura X, Zatelli A, Zini E.

Department of Veterinary Pathology, Hygiene and Public Health, School of Veterinary Medicine, University of Milan, 20122 Milan, Italy.

PMID: 20513195 [PubMed - in process]
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5. Emerg Infect Dis. 2010 Mar;16(3):400-5.

Infection of kissing bugs with Trypanosoma cruzi, Tucson, Arizona, USA.

Reisenman CE, Lawrence G, Guerenstein PG, Gregory T, Dotson E, Hildebrand JG.

Department of Neuroscience, College of Science, University of Arizona, Tucson, Arizona 85721-0077, USA. carolina@neurobio.arizona.edu

Abstract

Triatomine insects (Hemiptera: Reduviidae), commonly known as kissing bugs, are a potential health problem in the southwestern United States as possible vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although this disease has been traditionally restricted to Latin America, a small number of vector-transmitted autochthonous US cases have been reported. Because triatomine bugs and infected mammalian reservoirs are plentiful in southern Arizona, we collected triatomines inside or around human houses in Tucson and analyzed the insects using molecular techniques to determine whether they were infected with T. cruzi. We found that 41.5% of collected bugs (n = 164) were infected with T. cruzi, and that 63% of the collection sites (n = 22) yielded >or=1 infected specimens. Although many factors may contribute to the lack of reported cases in Arizona, these results indicate that the risk for infection in this region may be higher than previously thought.

Free Article
PMID: 20202413 [PubMed - indexed for MEDLINE]
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Publication Types:

  • Research Support, Non-U.S. Gov't

MeSH Terms:

  • Animals
  • Arizona
  • Chagas Disease/parasitology
  • Chagas Disease/transmission*
  • DNA, Protozoan/analysis
  • Female
  • Housing
  • Humans
  • Insect Vectors/parasitology*
  • Male
  • Triatoma/parasitology*
  • Trypanosoma cruzi/classification
  • Trypanosoma cruzi/genetics
  • Trypanosoma cruzi/isolation & purification*

Substances:

  • DNA, Protozoan
6. Colloids Surf B Biointerfaces. 2010 May 1;77(1):12-7. Epub 2010 Jan 11.

Immunodiagnosis of Chagas disease: Synthesis of three latex-protein complexes containing different antigens of Trypanosoma cruzi.

Gonzalez VD, Garcia VS, Vega JR, Marcipar IS, Meira GR, Gugliotta LM.

INTEC, Universidad Nacional del Litoral and CONICET, Güemes, Santa Fe, Argentina.

Abstract

This article describes the physical adsorption and the chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes to be used as novel immunodiagnosis reagents for detecting the Chagas disease. The coupled proteins were a homogenate of T. cruzi, or a recombinant protein (either Ag36 or CP1). With the homogenate, between 30 and 60% of the total-linked protein was chemically coupled, showing a small dependence with the pH. For Ag36 and CP1, around 90% of the total-linked protein was chemically coupled, with a maximum coupling at pH 5 (i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36. 2010 Elsevier B.V. All rights reserved.

PMID: 20137901 [PubMed - indexed for MEDLINE]
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Publication Types:

  • Research Support, Non-U.S. Gov't

MeSH Terms:

  • Antigens, Protozoan/analysis*
  • Chagas Disease/diagnosis*
  • Humans
  • Hydrogen-Ion Concentration
  • Isoelectric Point
  • Latex/chemistry*
  • Recombinant Proteins/chemical synthesis
  • Trypanosoma cruzi/immunology*

Substances:

  • Antigens, Protozoan
  • Latex
  • Recombinant Proteins

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