What's new for 'Trypanosomatids' in PubMed

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Sent on Thursday, 2010 Dec 16
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

Items 1 - 4 of 4

1.	Planta Med. 2010 Dec 14. [Epub ahead of print] Complete Structural Assignment of Serratol, a Cembrane-Type Diterpene from Boswellia serrata, and Evaluation of Its Antiprotozoal Activity. Schmidt TJ, Kaiser M, Brun R. Institut für Pharmazeutische Biologie und Phytochemie (IPBP), Westfälische Wilhelms-Universität Münster, Münster, Germany. Abstract From the dichloromethane extract obtained from the gum resin of BOSWELLIA SERRATA Roxb. (Burseraceae), a well-known medicinal plant resin ("Indian Olibanum"), the cembrane-type diterpene serratol was isolated in high yield. Its structure, previously reported without clear specification of double-bond geometry and without specification of stereochemistry, was reanalysed by means of spectroscopic measurements and unambiguously assigned as S(-)-cembra-3 E,7 E,11 E-triene-1-ol. Full assignment of all NMR data is reported for the first time. The compound was found to be identical with a cembrenol previously isolated from B. CARTERI. Serratol was tested for IN VITRO activity against four protozoan human pathogens, namely, TRYPANOSOMA BRUCEI RHODESIENSE (East African Human Trypanosomiasis, sleeping sickness), T. CRUZI (Chagas' disease), LEISHMANIA DONOVANI (Kala-Azar), and PLASMODIUM FALCIPARUM (Tropical Malaria). It was found active against T. BRUCEI and P. FALCIPARUM. These activities were 10- to 15-fold higher than its cytotoxicity against rat skeletal myoblasts. While some reports exist on potential anti-inflammatory activity of BOSWELLIA diterpenes, this is the first report on antiprotozoal activity of such a compound. © Georg Thieme Verlag KG Stuttgart · New York.
	PMID: 21157686 [PubMed - as supplied by publisher]

2.	Phytochemistry. 2010 Oct;71(14-15):1780-6. Epub 2010 Jul 23. Triterpenoidal alkaloids from Buxus hyrcana and their enzyme inhibitory, anti-fungal and anti-leishmanial activities. Ata A, Iverson CD, Kalhari KS, Akhter S, Betteridge J, Meshkatalsadat MH, Orhan I, Sener B. Department of Chemistry, The University of Winnipeg, Winnipeg, MB, Canada. a.ata@uwinnipeg.ca Abstract From the aerial parts of Buxus hyrcana, three triterpenoidal alkaloids, 17-oxo-3-benzoylbuxadine (1), buxhyrcamine (2), and 31-demethylcyclobuxoviridine (3), along with 16 known compounds, cyclobuxoviridine (4), N(b)-dimethylcyclobuxoviricine (5), E-buxenone (6), Z-buxenone (7), moenjodaramine (8), homomoenjodarmine (9), buxamine A (10), buxamine B (11), 31-hydroxybuxamine B (12), N(20)-formylbuxaminol E (13), papillozine C (14), buxmicrophylline F (15), buxrugulosamine (16), cyclobuxophylline O (17), spirofornabuxine (18) and arbora-1,9(11)-dien-3-one (19) were isolated. Their structures were elucidated by using NMR spectroscopic methods. All of the compounds exhibited moderate to weak acetylcholinesterase, butyrylcholinesterase and glutathione S-transferase inhibitory activities. Compounds 1-19 also exhibited modest anti-fungal activities against Candidaalbicans. Compounds 1, 2, 8, 9 and 18 also exhibited weak anti-leishmanial activity. Copyright © 2010 Elsevier Ltd. All rights reserved.
	PMID: 20655557 [PubMed - indexed for MEDLINE]
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3.	Toxicon. 2010 Nov;56(6):944-55. Epub 2010 Jul 6. L-amino acid oxidase activity present in fractions of Bothrops jararaca venom is respo nsible for the induction of programmed cell death in Trypanosoma cruzi. Deolindo P, Teixeira-Ferreira AS, DaMatta RA, Alves EW. Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28013-600 Campos dos Goytacazes, RJ, Brazil. Abstract Bothrops jararaca venom induces programmed cell death in epimastigotes of Trypanosoma cruzi. Here we fractionated the venom and observed that the anti-T. cruzi activity was associated with fractions that present L-amino acid oxidase (L-AAO) activity. L-AAO produces H(2)O(2), which is highly toxic. The addition of catalase to the medium, a H(2)O(2) scavenger, reverted the killing capacity of venom fractions. The anti-T. cruzi activity was also abolished when parasites were cultured in a medium without hydrophobic amino acids that are essential for L-AAO activity. These results were confirmed with a commercial purified L-AAO. Treatment for 24 h with fractions that present L-AAO activity induced parasites cytoplasmic retraction, mitochondrial swelling and DNA fragmentation, all morphological characteristics of programmed cell death. Similar changes were also observed when parasites were treated with H(2)O(2). These results indicate that H(2)O(2), the product of L-AAO reaction, induces programmed cell death explaining the anti-T. cruzi activity of B. jararaca venom. (c) 2010 Elsevier Ltd. All rights reserved.
	PMID: 20615423 [PubMed - indexed for MEDLINE]
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4.	Parasitol Res. 2010 Sep;107(4):855-63. Epub 2010 Jun 29. Microsatellite marker analysis shows differentiation among Trypanosoma cruzi p opulations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patients : microsatellite marker analysis and T. cruzi. Venegas J, Miranda S, Coñoepan W, Pîchuantes S, Jercic MI, González C, Gajardo M, Apt W, Arribada A, Sánchez G. Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile. jvenega@med.uchile.cl Abstract To investigate whether Trypanosoma cruzi populations found in chagasic cardiopathic and non-cardiopathic patients are genetically differentiated, three molecular microsatellite markers were analysed. This analysis was also applied to compare T. cruzi samples from peripheral blood or dejections of Triatoma infestans fed on the blood of the same patients. In order to obtain the first objective, analyses of predominant T. cruzi genotypes were conducted using three approaches: a locus-by-locus analysis; a Fisher method across three loci; and analysis of molecular variance by Genepop and Arlequin programs. Only with one locus and on the blood samples was a significant differentiation detected among non-cardiopathic and cardiopathic groups, which was not confirmed by the other two methods. On the contrary, with the three approaches, it was found that T. cruzi clones present in the blood of patients are genetically differentiated from those detected in dejections of T. infestans fed on the same patients. Our results showed that the most frequent lineage both in blood as well as in triatomine dejection samples was TcI. No significant difference in T. cruzi lineage distribution was observed among chagasic cardiopathic and non-cardiopathic patients. The majority of the samples (50-60%) had only one T. cruzi clone (uniclonal) either in blood or dejection samples.
	PMID: 20585804 [PubMed - indexed for MEDLINE]
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