What's new for 'Trypanosomatids' in PubMed

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Sent on Wednesday, 2011 Jan 26
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

Items 1 - 3 of 3

1.	Jpn J Radiol. 2011 Jan;29(1):59-62. Epub 2011 Jan 26. Multidetector computed tomography aspects of tracheal mucosal leishmaniasis localization. Viglianesi A, Di Mauro D, Petrillo G. Department of Radiology, Policlinic Hospital of University of Catania, Via Santa Sofia 78, Catania, 95123, Italy, a.viglianesi@hotmail.it. Abstract Tracheal mucosal localization of Leishmania is considered a rare and dangerous event. A single case of leishmaniasis of the trachea has been described in literature. Our work describes multidetector computed tomography features of leishmaniasis localized in the tracheal mucosa that occurred in a 68-year-old patient who lived in an endemic zone. Our patient underwent biopsy that established the diagnosis of leishmaniasis.
	PMID: 21264663 [PubMed - as supplied by publisher]
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2.	PLoS One. 2011 Jan 14;6(1):e16156. Mitochondrial Associated Ubiquitin Fold Modifier-1 Mediated Protein Conjugation in Leishmania donovani. Gannavaram S, Sharma P, Duncan RC, Salotra P, Nakhasi HL. Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland, United States of America. Abstract In this report, we demonstrate the existence of the ubiquitin fold modifier-1 (Ufm1) and its conjugation pathway in trypanosomatid parasite Leishmania donovani. LdUfm1 is activated by E1-like enzyme LdUba5. LdUfc1 (E2) specifically interacted with LdUfm1 and LdUba5 to conjugate LdUfm1 to proteinaceous targets. Mass spectrometry analysis revealed that LdUfm1 is conjugated to Leishmania protein targets that are associated with mitochondria. Immunofluorescence experiments showed that Leishmania Ufm1, Uba5 and Ufc1 are associated with the mitochondria. The demonstration that all the components of this system as well as the substrates are associated with mitochondrion suggests it may have physiological roles not yet described in any other organism. Overexpression of a non-conjugatable form of LdUfm1 and an active site mutant of LdUba5 resulted in reduced survival of Leishmania in the macrophage. Since mitochondrial activities are developmentally regulated in the life cycle of trypanosomatids, Ufm1 mediated modifications of mitochondrial proteins may be important in such regulation. Thus, Ufm1 conjugation pathway in Leishmania could be explored as a potential drug target in the control of Leishmaniasis.
	PMID: 21264253 [PubMed - as supplied by publisher]
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3.	Peptides. 2011 Jan 21. [Epub ahead of print] LEISHMANICIDAL ACTIVITY OF SYNTHETIC ANTIMICROBIAL PEPTIDES IN AN INFECTION MODEL WITH HUMAN DENDRITIC CELLS. Pérez-Cordero JJ, Lozano JM, Cortés J, Delgado G. Immunotoxicology Research Group, Department of Pharmacy, Faculty of Sciences, Universidad Nacional de Colombia, Bogotá, Colombia; Master of Science Program in Biochemistry, Department of Biochemistry, Faculty of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia. Abstract Different species of Leishmania are responsible for cutaneous, mucocutaneous or visceral leishmaniasis infections in millions of people around the world [14]. The adverse reactions caused by antileishmanial drugs, emergence of resistance and lack of a vaccine have motivated the search for new therapeutic options to control this disease. Different sources of antimicrobial molecules are under study as antileishmanial agents, including peptides with antimicrobial and/or immunomodulatory activity, which have been considered to be potentially active against diverse species of Leishmania [7,39]. This study evaluated the cytotoxicity on dendritic cells, hemolytic activity, leishmanicidal properties on Leishmania panamensis and Leishmania major promastigotes and effectiveness on parasite intracellular forms (dendritic cells infected with Leishmania panamensis and Leishmania major promastigotes), when each parasite in culture was exposed to different concentrations of a group of synthetic peptides with previously reported antimicrobial properties, which were synthesized based on their naturally occurring reported sequences. Dermaseptin, Pr-2 and Pr-3 showed inhibitory activity on the growth of Leishmania panamensis promastigotes, while Andropin and Cecropin A (with a selectivity index of 4 and 40, respectively) showed specific activity against intracellular forms of this species. The activities of Andropin and Cecropin A were exclusively against the intracellular forms of the parasite, therefore indicating the relevance of these two peptides as potential antileishmanial agents. In the case of Leishmania major promastigotes, Melittin and Dermaseptin showed inhibirtory activity, the latter also showed a selectivity index of 8 against intracellular forms. These findings suggest Andropin, Cecropin A and Dermaseptin as potential therapeutic tools to treat New and Old World cutaneous leishmaniasis. Copyright © 2011. Published by Elsevier Inc.
	PMID: 21262294 [PubMed - as supplied by publisher]
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