What's new for 'Trypanosomatids' in PubMed

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Sent on Friday, 2011 Oct 21
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

Items 1 - 5 of 5

1.	Rev Inst Med Trop Sao Paulo. 2011 Oct;53(5):283-9. Use of elisa employing homologous and heterologous antigens for the detection of IgG and subclasses (IgG1 and IgG2) in the diagnosis of Canine visceral leishmaniasis. Ribeiro FC, Schubach Ade O, Mouta-Confort E, Pacheco TM, Madeira Mde F, Abboud LC, Honse Cde O, Alves AS, Marzochi MC. Source Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. Abstract Indirect immunofluorescence is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is limited. Since ELISA does not present these limitations, this technique might be an option for the detection of IgG or specific IgG1 and IgG2 subclasses. Canine ehrlichiosis is an important differential diagnosis of American Visceral Leishmaniasis (AVL). The present study compared ELISA using Leishmania chagasi and Leishmania braziliensis antigen for the detection of anti-Leishmania IgG and subclasses in serum samples from 37 dogs naturally infected with L. chagasi (AVL) and in samples from four dogs co-infected with L. braziliensis and L. chagasi (CI). The occurrence of cross-reactivity was investigated in control serum samples of 17 healthy dogs (HC) and 35 infected with Ehrlichia canis (EC). The mean optical density obtained for the detection of IgG was significantly higher when L. chagasi antigen was used, and was also higher in subgroup VLs (symptomatic) compared to subgroup Vla (asymptomatic). The correlation between IgG and IgG1 was low. The present results suggest that IgG ELISA using homologous antigen yields the best results, permitting the diagnosis of asymptomatic L. chagasi infection and the discrimination between cases of AVL and ehrlichiosis in dogs.
	PMID: 22012455 [PubMed - in process]

2.	Rev Inst Med Trop Sao Paulo. 2011 Oct;53(5):255-8. Sandfly fauna (Diptera: Psychodidae) in Parque do Sabiá complex, Uberlândia, Minas Gerais, Brazil. Rodrigues Ede A, Andrade Filho JD, Limongi JE, Paula MB. Source Centro de Controle de Zoonoses, Uberlândia, MG, Brazil. Abstract Between April 2003 and May 2009 phlebotomine sandflies were collected in Parque do Sabiá complex, Uberlândia municipality, Minas Gerais State, Brazil, using CDC and Shannon traps. The objective was to associate the sandfly species captured to the risk of the transmission of leishmaniasis in the municipality. The 126 captured specimens belonging to six species of phlebotomine, among which Lutzomyia (Psychodopygus) davisi (Root, 1934) predominated with 113 specimens (89.7%). The remaining captured species were Lutzomyia (Pintomyia) mamedei Oliveira, Afonso, Dias & Brazil, 1994 - five specimens (3.9%); Lutzomyia (Nyssomyia) flaviscutellata (Mangabeira, 1942) - four specimens (3.2%); Lutzomyia lenti (Mangabeira, 1938) - two specimens (1.6%); Brumptomyia avellari (Costa Lima, 1932) - one specimen (0.8%); and Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho, 1939) - one specimen (0.8%). The collection of species that may be involved in the transmission of Leishmania reveals the need for continuous entomological surveillance.
	PMID: 22012450 [PubMed - in process]

3.	Mem Inst Oswaldo Cruz. 2011 Sep;106(6):731-4. How to increase the population of a Phlebotomus perniciosus (Diptera: Psychodidae) colony: a new method. Alarcón-Elbal PM, Montoliu BG, Pinal R, Delacour-Estrella S, Ruiz-Arrondo I, Peribáñez MÁ, Blas ID, Molina R, Castillo JA, Diéguez-Fernández L, Lucientes J. Abstract The sandfly Phlebotomus perniciosus is the most widespread vector of Leishmania infantum in Spain. Laboratory colonisation represents the most feasible source of information on the biology of these insects, but in conducting any study, the density of individuals in the colony may drop to such an extent that it is sometimes difficult to recover the initial population levels. A new technique was tested for the recovery of sandfly eggs in three different colonies; the recovery rate was studied by comparing the standard method of mass rearing with this new method of colony management. The results demonstrate a mean increase of 18.4% in adult production, a growth in colony productivity that justifies the inclusion of this process in the routine maintenance of any colony of sandflies.
	PMID: 22012228 [PubMed - in process]

4.	J Invest Dermatol. 2011 Oct 20. doi: 10.1038/jid.2011.291. [Epub ahead of print] Exposure to Phlebotomus papatasi and/or Leishmania major: Possible Etiologic Link to Tunisian Pemphigus. Zaraa I, Boussoffara T, Ben Ahmed M, Marzouki S, Ben Hassouna N, Kallel Sellami M, Makni S, Ben Osman A, Louzir H, Mokni M. Source 1] Department of Dermatology, Hospital La Rabta, Tunis, Tunisia [2] Faculté de Médecine de Tunis, Tunis El Manar University, Tunis, Tunisia [3] Research Unit UR 24/04, Tunis, Tunisia.
	PMID: 22011908 [PubMed - as supplied by publisher]

5.	Infect Agent Cancer. 2011 Oct 20;6(1):17. [Epub ahead of print] Rare association of Visceral leishmaniasis with Hodgkin's disease: A case report. Kumar R, Daga MK, Kamble NL, Sothwal A, Singh T, Nayak HK, Raizada N. Abstract ABSTRACT: We present here a case of young male with complaints of fever and swelling in neck for eight months. History of progressive weakness associated with weight loss was present. Physical examination revealed pallor, multiple enlarged cervical lymph nodes and hepatosplenomegaly. Investigations showed pancytopenia, hyperglobinemia and Leishman-Donovan bodies on bone marrow aspiration. Serological test confirmed diagnosis of visceral leishmaniasis. However, cervical lymph node aspiration and biopsy were suggestive of Mixed cellularity Hodgkin's disease. This made it a very rare case of leishmaniasis as an opportunistic infection in a patient of pre-chemotherapy Hodgkin's disease. There was marked improvement in haematological profile and regression of hepatosplenomegaly with Amphotericin B treatment followed by favourable response to chemotherapy. The case emphasizes the suspicion for leishmaniasis as a masquerader and as an opportunistic infection in haematological malignancies.
	PMID: 22011565 [PubMed - as supplied by publisher]