Friday, February 27, 2009

What's new for 'Trypanosomatids' in PubMed

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Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results
Items 1 -2 of 2

1: Trans R Soc Trop Med Hyg. 2008 Nov;102(11):1102-7. Epub 2008 Jul 9.Click here to read LinkOut

kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients.

Manoel-Caetano Fda S, Carareto CM, Borim AA, Miyazaki K, Silva AE.

Department of Biology, São Paulo State University, São José do Rio Preto, SP, Brazil.

Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T. cruzi kDNA minicircle gene signatures using the low-stringency single-specific-primer PCR technique in both peripheral blood and oesophageal mucosa from chronic chagasic patients, with or without megaesophagus, alone or in combination with cardiopathy and megacolon. It was not possible to identify a uniform pattern of shared bands between blood and oesophageal mucosa samples from individuals with the same clinical form or mixed forms, suggesting multiple T. cruzi infections with differential tissue tropism. Thus, the results indicate that there is an intense intraspecific variability in the hypervariable regions of T. cruzi kDNA, which has so far made it impossible to correlate the genetic profile of this structure with the clinical manifestations of Chagas disease.

PMID: 18617206 [PubMed - indexed for MEDLINE]

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2: Trans R Soc Trop Med Hyg. 2008 Nov;102(11):1133-9. Epub 2008 Jun 9.Click here to read LinkOut

The coati (Nasua nasua, Carnivora, Procyonidae) as a reservoir host for the main lineages of Trypanosoma cruzi in the Pantanal region, Brazil.

Herrera HM, Lisboa CV, Pinho AP, Olifiers N, Bianchi RC, Rocha FL, Mourão GM, Jansen AM.

Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, Rio de Janeiro, RJ, 21040-900, Brazil.

We have focused on the role played by a carnivore, the coati (Nasua nasua), in the transmission cycle of Trypanosoma cruzi in the Brazilian Pantanal biome. We collected data during 2000/01 and 2005-07. Prevalence and pattern of T. cruzi infection were determined by serological tests and hemoculture. Isolates were characterized by miniexon molecular assay. Our results demonstrate that T. cruzi transmission cycle among coatis in the southern Pantanal seems to be well established, as we found high serum prevalences and high parasitemias throughout the two studied periods. Single infections by TCII (32.1%), TCI (28.0%) and Z3 (7.1%) were observed. Mixed infections by TCI/TCII (10.7%) and TCI/Z3 (3.6%) were also detected. Distinct genotypes of T. cruzi could be recovered during the 8 months follow-up of the same animals. As free-living coatis have high densities and inhabit all habitats, they may play an important role in the maintenance and dispersion of the main T. cruzi subpopulations. Considering that the Pantanal connects some of the major biomes of South America, it may be acting as a corridor for the spread of the main T. cruzi subpopulations. Our data give support that predator-prey links are important mechanisms for T. cruzi transmission and perpetuation in the wild.

PMID: 18541281 [PubMed - indexed for MEDLINE]

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