This message contains My NCBI what's new results from the National Center for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM).
Do not reply directly to this message.
Sender's message:
Sent on Wednesday, 2010 Feb 10Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
Click here to view complete results in PubMed. (Results may change over time.)
To unsubscribe from these e-mail updates click here.
| PubMed Results |
| 1. | Parasitol Res. 2010 Feb 9. [Epub ahead of print]Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei.Verner Z, Paris Z, Lukeš J.Biology Centre, Institute of Parasitology, Czech Academy of Sciences, and Faculty of Sciences, University of South Bohemia, 37005, Ceské Budejovice (Budweis), Czech Republic. We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential. |
| PMID: 20143094 [PubMed - as supplied by publisher] | |
| Related articles | |
| 2. | Parasitol Res. 2010 Feb 9. [Epub ahead of print]A cross-sectional study of trypanosomosis and its vectors in donkeys and mules in Northwest Ethiopia.Abebe R, Wolde A.Faculty of Veterinary Medicine, Hawassa University, P.O. Box 05, Hawassa, Ethiopia, rahmetoa@yahoo.com. A preliminary study was conducted in January 2009 in four peasant associations (PAs) selected from two districts in Benishangul Gumuz Regional State, Northwest Ethiopia to investigate the prevalence and species of trypanosomes infecting donkeys and mules and identify the fly vectors playing a role in the transmission of trypanosomosis. Blood samples were collected from a total of 334 donkeys and 52 mules and examined by dark ground/phase contrast buffy coat technique and Giemsa-stained blood smears. Accordingly, trypanosome species were encountered in 6.3% of the examined donkeys (n = 21) while none of the mules examined was positive for trypanosome infection. Trypanosomes and tsetse flies were detected in two of the four PAs surveyed (Tsetsa adurno and Bamadone) with significant (P = 0.004) difference in prevalence. The inability to find trypanosomes in the other two PAs (Ura and Ashura) was most likely due to the absence of appropriate fly vectors. Three species of trypanosomes were detected in donkeys, which in order of predominance were Trypanosoma congolense (52.4%), Trypanosoma brucei (28.6%), and Trypanosoma vivax (19.05%). There was a significant (P = 0.008) difference in mean PCV between trypanosome infected and non-infected donkeys. The body condition score of the donkeys was significantly associated with both prevalence of infection (P = 0.009) and mean packed cell volume (PCV; P < 0.0001). No significant difference was observed between male and female donkeys regarding both prevalence of infection and mean PCV (P > 0.05 for each factor). The entomological surveys revealed the presence of Glossina morsitans submorsitans and other biting flies of the family Stomoxys, Tabanus, and Haematopota. In conclusion, the prevalence of trypanosomosis obtained in the current study is generally low compared to previous studies. As the present study design was a cross-sectional, one that only depicts a momentary picture of the infection status in the herd, a further longitudinal study that makes use of more sensitive techniques and entomological survey is recommended. |
| PMID: 20143093 [PubMed - as supplied by publisher] | |
| Related articles | |
| 3. | J Coll Physicians Surg Pak. 2010 Jan;20(1):65-7.Rehabilitaton of nose using silicone based maxillofacial prosthesis.Ahmed B, Butt AM, Hussain M, Amin M, Yazdanie N.Department of Prosthodontics and Oral Implantology, University of Faisalabad, Faisalabad. Surgical reconstruction of large facial defects is not possible at times due to extensive loss of tissue that cannot be corrected by surgery alone. In these cases, prosthetic restoration of lost facial tissue can be done using maxillofacial prosthetics. Introduction of new material which gives life-like appearance to such prosthetic restorations e.g. silicone and poly ether rubbers and use of implants to retain these prostheses have given a new dimension to rehabilitation of such patients. This report presents a case of prosthetic rehabilitation of the nasal component of the face secondary to nasal leishmaniasis, using precision attachment retained maxillofacial prosthesis. |
| PMID: 20141699 [PubMed - in process] | |
| Related articles | |
| 4. | Biochem J. 2010 Jan 15;425(3):513-22.Structural insights into the catalytic mechanism of Trypanosoma cruzi GPXI (glutathione peroxidase-like enzyme I).Patel S, Hussain S, Harris R, Sardiwal S, Kelly JM, Wilkinson SR, Driscoll PC, Djordjevic S.Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London WC1E 6BT, UK. s.djordjevic@ucl.ac.uk Current drug therapies against Trypanosoma cruzi, the causative agent of Chagas disease, have limited effectiveness and are highly toxic. T. cruzi-specific metabolic pathways that utilize trypanothione for the reduction of peroxides are being explored as potential novel therapeutic targets. In the present study we solved the X-ray crystal structure of one of the T. cruzi enzymes involved in peroxide reduction, the glutathione peroxidase-like enzyme TcGPXI (T. cruzi glutathione peroxidase-like enzyme I). We also characterized the wild-type, C48G and C96G variants of TcGPXI by NMR spectroscopy and biochemical assays. Our results show that residues Cys48 and Cys96 are required for catalytic activity. In solution, the TcGPXI molecule readily forms a Cys48-Cys96 disulfide bridge and the polypeptide segment containing Cys96 lacks regular secondary structure. NMR spectra of the reduced TcGPXI are indicative of a protein that undergoes widespread conformational exchange on an intermediate time scale. Despite the absence of the disulfide bond, the active site mutant proteins acquired an oxidized-like conformation as judged from their NMR spectra. The protein that was used for crystallization was pre-oxidized by t-butyl hydroperoxide; however, the electron density maps clearly showed that the active site cysteine residues are in the reduced thiol form, indicative of X-ray-induced reduction. Our crystallographic and solution studies suggest a level of structural plasticity in TcGPXI consistent with the requirement of the atypical two-cysteine (2-Cys) peroxiredoxin-like mechanism implied by the behaviour of the Cys48 and Cys96 mutant proteins. |
| PMID: 19886864 [PubMed - indexed for MEDLINE] | |
| Related articles | |
 | |
MeSH Terms:
Substances:
|
No comments:
Post a Comment