This message contains My NCBI what's new results from the National Center for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM).
Do not reply directly to this message.
Sender's message:
Sent on Thursday, 2010 Mar 04Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
Click here to view complete results in PubMed. (Results may change over time.)
To unsubscribe from these e-mail updates click here.
| PubMed Results |
| 1. | Vet Parasitol. 2010 Feb 12. [Epub ahead of print]A seroepidemiological study of exposure to Toxoplasma, Leishmania, Echinococcus and Trichinella in equids in Greece and analysis of risk factors.Kouam MK, Diakou A, Kanzoura V, Papadopoulos E, Gajadhar AA, Theodoropoulos G.Department of Anatomy and Physiology of Farm Animals, Faculty of Animal Science and Hydrobiology, Agricultural University of Athens, 75 Iera Odos, Votanikos, Athens 11855, Greece. The role of horses in the transmission of parasitic zoonoses either as a source of infection to vectors or through contamination of definitive hosts is gaining importance worldwide. For this reason sera from 773 equids including 753 horses, 13 mules and seven ponies in four regions of Greece were investigated by ELISA for the presence of IgG antibodies against Toxoplasma, Leishmania, Echinococcus and Trichinella. Anti-Toxoplasma antibodies were detected in all regions with an overall prevalence of 1.8%. In contrast, antibodies to Leishmania, Echinococcus, and Trichinella were present only in horses from the equestrian centre located in Attica region, but the status of Trichinella could not be confirmed. The seroprevalence of infection was 0.3% for Leishmania, 0.1% for Echinococcus and 0.1% for Trichinella. Only one horse was positive with a mixed infection of Toxoplasma, Leishmania and Trichinella. The following host characteristics were investigated for any significant effects on the prevalence of Toxoplasma infection: gender, age, species, origin of birth, activity, and location. The type of activity (p<0.05) and location (p<0.01) of the animals were found to be significant risk factors for Toxoplasma infection. The relative risk (RR) for Toxoplasma infection comparing the regions of Peloponnese and Thessaly to Attica were 6.92 and 6.78, respectively. Due to the very low prevalence of Echinococcus, Leishmania, and Trichinella infections, the associated risk factors were not analysed. The low seroprevalences observed suggest that the risk of infection from equids to people is very low, especially when consumption of horse meat is uncommon in this country. Copyright © 2010 Elsevier B.V. All rights reserved. |
| PMID: 20197215 [PubMed - as supplied by publisher] | |
| 2. | Microbes Infect. 2010 Feb 27. [Epub ahead of print]Scrutinizing the mechanisms underlying the induction of anemia of inflammation through GPI-mediated modulation of macrophage activation in a model of African trypanosomiasis.Stijlemans B, Vankrunkelsven A, Brys L, Raes G, Magez S, De Baetselier P.Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel (VUB),1050 Brussels, Belgium. In animal trypanosomiasis the severity of infection is reflected by the degree of anemia which resembles anemia of inflammation, involving a skewed iron-homeostasis leading to iron-accumulation within the reticuloendothelial system. Myeloid cells (M cells) have been implicated in the induction and maintenance of this type of anemia and modulation of M cells through the main trypanosome-derived glycosylphosphatidyl inositol (GPI)-anchor could attenuate both anemia and trypano-susceptiblity in T. brucei infected mice. Herein the GPI-based treatment, allowing a straightforward comparison between trypanotolerance and susceptibility in T. brucei infected C57Bl/6 mice, was further adopted to scrutinize mechanisms/pathways underlying trypanosome-elicited anemia. Hereby, the following interlinkable observations were made in GPI-based treated (GBT) T. brucei infected mice: (i) a reduced inflammatory cytokine production and increased IL-10 production associated with alleviation of anemia and restoration of serum iron levels, (ii) a shift in increased liver expression of iron storage towards iron export genes, (iii) increased erythropoiesis in the bone-marrow and extra-medullar sites (spleen) probably reflecting a normalized iron homeostasis and availability. Collectively, our results demonstrate that reprogramming macrophages towards an anti-inflammatory state alleviates anemia of inflammation by normalizing iron homeostasis and restoring erythropoiesis. Copyright © 2010 Elsevier Masson SAS. All rights reserved. |
| PMID: 20197106 [PubMed - as supplied by publisher] | |
| 3. | Parasit Vectors. 2010 Mar 2;3(1):13. [Epub ahead of print]Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis.Mugasa CM, Laurent T, Schoone GJ, Basiye FL, Saad AA, El Safi S, Kager PA, Schallig HD.ABSTRACT: BACKGROUND: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n=30) and cutaneous leishmaniasis from South America (n=70) and appropriate control samples. RESULTS: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. CONCLUSION: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer. |
| PMID: 20196849 [PubMed - as supplied by publisher] | |
| 4. | Gene. 2010 Mar 1;452(2):72-8. Epub 2010 Jan 7.Proteomic analysis reveals the dynamic association of proteins with translated mRNAs in Trypanosoma cruzi.Alves LR, Avila AR, Correa A, Holetz FB, Mansur FC, Manque PA, de Menezes JP, Buck GA, Krieger MA, Goldenberg S.Instituto Carlos Chagas, Laboratório de Regulação da Expressão Gênica, Curitiba, Paraná, Brazil. Gene regulation is mainly post-transcriptional in trypanosomatids. The stability of mRNA and access to polysomes are thought to be tightly regulated, allowing Trypanosoma cruzi to adapt to the different environmental conditions during its life cycle. Post-transcriptional regulation requires the association between mRNAs and certain proteins to form mRNP complexes. We investigated the dynamic association between proteins and mRNAs, using poly(T) beads to isolate and characterize proteins and protein complexes bound to poly-A+ mRNAs. The protein content of these fractions was analyzed by mass spectrometry (LC-MS/MS). We identified 542 protein component of the mRNP complexes associated with mRNAs. Twenty-four of the proteins obtained were present in all fractions, whereas some other proteins were exclusive to a particular fraction: epimastigote polysomal (0.37%) and post-polysomal (2.95%) fractions; stress polysomal (13.8%) and post-polysomal (40.78%) fractions. Several proteins known to be involved in mRNA metabolism were identified, and this was considered important as it made it possible to confirm the reliability of our mRNP isolation approach. This procedure allowed us to have a first insight into the composition and dynamics of mRNPs in T. cruzi. Copyright 2009 Elsevier B.V. All rights reserved. |
| PMID: 20060445 [PubMed - indexed for MEDLINE] | |
| Related articles | |
 | |
Publication Types:
MeSH Terms:
Substances:
|
| 5. | Infect Immun. 2010 Feb;78(2):810-22. Epub 2009 Nov 16.Genetic immunization converts the trypanosoma cruzi B-Cell mitogen proline racemase to an effective immunogen.Bryan MA, Norris KA.Department of Immunology, University of Pittsburgh School of Medicine, BST E1038, 200 Lothrop Street, Pittsburgh, PA 15213, USA. Trypanosoma cruzi is the etiologic agent of Chagas' disease. Acute T. cruzi infection results in polyclonal B-cell activation and delayed specific humoral immunity. T. cruzi proline racemase (TcPRAC), a T. cruzi B-cell mitogen, may contribute to this dysfunctional humoral response. Stimulation of murine splenocytes with recombinant protein (rTcPRAC) induced B-cell proliferation, antibody secretion, interleukin-10 (IL-10) production, and upregulation of CD69 and CD86 on B cells. Marginal zone (MZ) B cells are more responsive to T-cell-independent (TI) rTcPRAC stimulation than are follicular mature (FM) B cells in terms of proliferation, antibody secretion, and IL-10 production. During experimental T. cruzi infection, TcPRAC-specific IgG remained undetectable when responses to other T. cruzi antigens developed. Conversely, intradermal genetic immunization via gene gun (GG) delivered TcPRAC as an immunogen, generating high-titer TcPRAC-specific IgG without B-cell dysfunction. TcPRAC GG immunization led to antigen-specific splenic memory B-cell and bone marrow plasma cell formation. TcPRAC-specific IgG bound mitogenic rTcPRAC, decreasing subsequent B-cell activation. GG immunization with rTcPRAC DNA was nonmitogenic and did not affect the generation of specific IgG to another T. cruzi antigen, complement regulatory protein (CRP). These data demonstrate the utility of genetic immunization for the conversion of a protein mitogen to an effective antigen. Furthermore, coimmunization of TcPRAC with another T. cruzi antigen indicates the usefulness of this approach for multivalent vaccine development. PMCID: PMC2812217 [Available on 2010/8/1] |
| PMID: 19917711 [PubMed - indexed for MEDLINE] | |
| Related articles | |
 | |
Publication Types:
MeSH Terms:
Substances:
Grant Support:
|
| 6. | An Acad Bras Cienc. 2009 Sep;81(3):477-88.Current relevance of fungal and trypanosomatid glycolipids and sphingolipids: studies defining structures conspicuously absent in mammals.Takahashi HK, Toledo MS, Suzuki E, Tagliari L, Straus AH.Setor de Imunoquímica de Glicoconjugados, Departamento de Bioquímica, Ed. J.L. Prado, Rua Botucatu, 862, 04023-900 São Paulo, SP, Brasil. Recently, glycosphingolipids have been attracting attention due to their role on biological systems as second messengers or modulators of signal transduction, affecting several events, which range from apoptosis to regulation of the cell cycle. In pathogenic fungi, glycolipids are expressed in two classes: neutral monohexosylceramides (glucosyl-or galactosylceramide) and acidic glycosylinositol phosphorylceramides (the latter class carries longer glycan chains). It is worth to mention that monohexosylceramides exhibit significant structural differences in their lipid moieties compared to their mammalian counterparts, whereas the glycosylinositol phosphorylceramides exhibit remarkable structural differences in their carbohydrate moieties in comparison to mammal glycosphingolipids counterpart. We observed that glycosylinositol phosphorylceramides are capable of promoting immune response in infected humans. In addition, inhibiting fungal glycosphingolipid biosynthetic pathways leads to an inhibition of colony formation, spore germination, cell cycle, dimorphism and hyphal growth. Other pathogens, such as trypanosomatids, also present unique glycolipids, which may have an important role for the parasite development and/or disease establishment. Regarding host-pathogen interaction, cell membrane rafts, which are enriched in sphingolipids and sterols, participate in parasite/fungal infection. In this review, it is discussed the different biological roles of (glyco) (sphingo)lipids of pathogenic/opportunistic fungi and trypanosomatids. |
| PMID: 19722017 [PubMed - indexed for MEDLINE] | |
| Related articles Free article | |
 | |
Publication Types:
MeSH Terms:
Substances:
|
No comments:
Post a Comment