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Sent on Wednesday, 2010 Apr 28Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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| PubMed Results |
| 1. | J Biol Chem. 2010 Apr 26. [Epub ahead of print]Plasmodium falciparum dynein light chain 1 interacts with actin/myosin during blood stage development.Daher W, Pierrot C, Kalamou H, Pinder JC, Margos G, Dive D, Franke-Fayard B, Janse CJ, Khalife J.Department of Microbiology and Molecular Medicine, CMU, University of Geneva, Switzerland; AbstractDynein light chain 1 (LC1), a member of the Leucine Rich Repeat protein family, has been shown to be engaged in controlling flagellar motility in Chlamydomonas reinhardtii and Trypanosoma brucei via its interaction with the dynein gamma heavy chain. In Plasmodium falciparum, we have identified the LC1 ortholog, designated PfDLC1. Negative attempts to disrupt the dlc1 gene by reverse genetic approaches in both P. falciparum and P. berghei suggest either its essentiality for parasite survival or the inaccessibility of its locus. Expression studies revealed high levels of DLC1 protein in late trophozoites and schizonts, pointing to an unexpected role of this protein in blood-stage parasites as they do not have flagella. Interactions studies and coimmunoprecipitation experiments revealed that PfDLC1 was able to bind to P. falciparum myosin A and actin 1. The PfDLC1 interacting domains present in P. falciparum myosin A and actin 1 were mapped to sequences containing SDIE and/or EEMKT motifs present in the upper 50 kDa segment of the myosin A head domain and in the subdomain IV of actin 1 respectively. Detection of PfDLC1 by fluorescence tagging and immunofluorescence staining using specific antibodies showed a cytoplasmic location similar to actin and immunofluorescence studies showed a colocalization of PfDLC1 and myosin A. Taken together, these findings suggest that PfDLC1 might play an important role in P. falciparum erythrocytic stages by its interaction with myosin A and actin 1, known to be essential for parasite development. |
| PMID: 20421304 [PubMed - as supplied by publisher] | |
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| 2. | Vector Borne Zoonotic Dis. 2010 Apr 26. [Epub ahead of print]Serological Study of Selected Vector-Borne Diseases in Shelter Dogs in Central Spain Using Point-of-Care Assays.Couto CG, Lorentzen L, Beall MJ, Shields J, Bertolone N, Couto JI, Couto KM, Nash S, Slack J, Kvitko H, Westendorf N, Marin L, Iazbik MC, Vicario FC, Sanz P, Ruano R.1 Department of Veterinary Clinical Sciences, The Ohio State University , Columbus, Ohio. AbstractAbstract We evaluated the prevalence of selected vector-borne diseases in 131 dogs in an animal shelter in central Spain using point-of-care assays (SNAP 4DX and SNAP Leishmania; IDEXX Laboratories, Westbrook, ME). The SNAP 4DX detects Dirofilaria immitis (Di) antigen and antibodies against Ehrlichia canis (Ec), Borrelia burgdorferi (Bb), and Anaplasma phagocytophylum (Aph); the SNAP Leishmania kit detects antibodies against Leishmania infantum (Li). Dogs were classified as healthy or sick based on physical examination, complete blood counts, and serum chemistry profiles. The prevalence of positive test results was as follows: Ec, 5.3% (n = 7); Aph, 19.0% (n = 25); Bb, 0%; Di, 0%; and Li, 5.3% (n = 7). Four dogs (3%) were coexposed to Ec and Aph, and three dogs (2.3%) were coexposed to Aph and Li. There was no statistically significant correlation between positive serology and clinical status (sick vs. healthy) or hematologic/biochemical abnormalities. The prevalence of Aph was the highest and is in agreement with a recent report in a dog shelter in northwestern Spain. These point-of-care assays may be more valuable as epidemiologic than as clinical tools. |
| PMID: 20420531 [PubMed - as supplied by publisher] | |
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| 3. | Vector Borne Zoonotic Dis. 2010 Apr 26. [Epub ahead of print]Genome Survey Sequence Analysis and Identification of Homologs of Major Surface Protease (gp63) Genes in Trypanosoma rangeli.Ferreira KA, Ruiz JC, Dias FC, Lages-Silva E, Tosi LR, Ramírez LE, Pedrosa AL.1 Disciplina de Biologia Molecular, Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro , Uberaba, Minas Gerais, Brazil . AbstractAbstract In this study, 222 genome survey sequences were generated for Trypanosoma rangeli strain P07 isolated from an opossum (Didelphis albiventris) in Minas Gerais State, Brazil. T. rangeli sequences were compared by BLASTX (Basic Local Alignment Search Tool X) analysis with the assembled contigs of Leishmania braziliensis, Leishmania infantum, Leishmania major, Trypanosoma brucei, and Trypanosoma cruzi. Results revealed that 82% (182/222) of the sequences were associated with predicted proteins described, whereas 18% (40/222) of the sequences did not show significant identity with sequences deposited in databases, suggesting that they may represent T. rangeli-specific sequences. Among the 182 predicted sequences, 179 (80.6%) had the highest similarity with T. cruzi, 2 (0.9%) with T. brucei, and 1 (0.5%) with L. braziliensis. Computer analysis permitted the identification of members of various gene families described for trypanosomatids in the genome of T. rangeli, such as trans-sialidases, mucin-associated surface proteins, and major surface proteases (MSP or gp63). This is the first report identifying sequences of the MSP family in T. rangeli. Multiple sequence alignments showed that the predicted MSP of T. rangeli presented the typical characteristics of metalloproteases, such as the presence of the HEXXH motif, which corresponds to a region previously associated with the catalytic site of the enzyme, and various cysteine and proline residues, which are conserved among MSPs of different trypanosomatid species. Reverse transcriptase-polymerase chain reaction analysis revealed the presence of MSP transcripts in epimastigote forms of T. rangeli. |
| PMID: 20420528 [PubMed - as supplied by publisher] | |
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| 4. | Nat Prod Commun. 2010 Mar;5(3):387-90.Leishmanicidal activity of racemic +/- 8-[(4-amino-1-methylbutyl)amino]-6-methoxy-4-methyl-5-[3,4-dichlorophenoxy]quinoline.Isaac-Márquez AP, McChesney JD, Nanayakara NP, Satoskar AR, Lezama-Dávila CM.Centro de Investigaciones en Enfermedades Tropicales, Universidad Autónoma de Campeche, Av. Patricio Trueba de Regil S/N, Campeche, Camp. 24090, México. anpisaac@hotmail.com AbstractIn this work we studied the in vitro toxicity of +/- 8-[(4-Amino-1-Methylbutyl)Amino]-6-Methoxy-4-Methyl-5-[3,4-dichlorophenoxy]quinoline (DN3-27-1) against stationary phase promastigotes Leishmania (L.) mexicana. Our results indicate that this drug induces an important reduction in parasite growth and killing compared to the reference drug N-methyl meglumine (Glucantime). DN3-27-1 was not toxic to Hela cells cultured in vitro. This is the first report describing the promising potential of DN3-27-1 in treatment of L. (L.) mexicana infections. |
| PMID: 20420313 [PubMed - in process] | |
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| 5. | Bull Soc Pathol Exot. 2009 Dec;102(5):352-5.[New type of human trypanosomiasis][Article in French] Chagas C; Société de pathologie exotique. |
| PMID: 20131433 [PubMed - indexed for MEDLINE] | |
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| 6. | Bull Soc Pathol Exot. 2009 Dec;102(5):319-25.[Chagas disease in chronic phase outside the endemic area. The diagnostic tools][Article in French] Paris L, Touafek F, Elghouzzi MH, Chérif S, Mazier D.Laboratoire de parasitologie-mycologie, groupe hospitalier Pitié-Salpêrière, 47, boulevard de l'Hôpital, F-75013 Paris, France. luc.paris@psl.ap-hop-paris.fr AbstractThe diagnosis of Chagas disease during the chronic phase is based on serology. Outside South America the use of two methods is recommended by WHO. A third method must be available for inconclusive results but there is no gold standard. A pilot study of screening in 254 Bolivian people living in the Paris area (France) was made. Serological study was performed using IIF and three Elisa, Elisa Cruzi (BioMérieux Brésil), BioElisa Chagas (Bio-kit), and Chagatest Elisa recombinante v. 3.0 (Wiener Lab). 165 patients were negative, 69 positive and 20 inconclusive. PCR-based assays appear to have a better sensitivity than parasitological methods, but not more than 70% that do not justify their use for primary testing. There are no standardized and commercial assays. The primer pairs based on the nuclear sequence TCZ1-TCZ2 seems to be the more specific (no cross reaction with others Trypanosomatidae) and the most sensitive with the strains of the two lineage of Trypanosoma cruzi. PCR would have a role in inconclusive serological cases or in the evaluation of treatment failure. |
| PMID: 20131426 [PubMed - indexed for MEDLINE] | |
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| 7. | Bull Soc Pathol Exot. 2009 Dec;102(5):300-9.[Screening for congenital infection by Trypanosoma cruzi in France][Article in French] Brutus L, Santalla JA, Salas NA, Schneider D, Chippaux JP.Institut de recherche pour le développement (IRD), UR010 "Santé de la mère et de l'enfant en milieu tropical", Université Paris-Descartes, 12, rue de l'Ecole de Médecine, F-75006 Paris, France. brutus@ird.fr AbstractThe importance of congenital transmission of Chagas' disease increases with its emergence in communities infected with Trypanosoma cruzi, but where vector transmission has never existed or is fully controlled through vector control campaigns. In both endemic and non-endemic areas, the rates of mother-to-child transmission (MTCT) could be the same, by 5%, generating a constant source of new cases of the disease. Risk factors for vertical transmission are not fully elucidated, but the effectiveness of the adaptive immune response and the genetic susceptibility of both the mother and the child are suspected. Besides the risk of miscarriage or premature birth, neonatal infection by T. cruzi causes an acute form of Chagas disease, which may be accompanied by a severe infectious syndrome that can causes death if not treated early. This form of the disease is a real public health priority because it is frequent, severe, identifiable and curable. Indeed, almost all newborns diagnosed and treated before the end of their first year of life will be definitely cured. In all non-endemic areas, detection of cases of congenital Chagas disease is hampered by a very low prevalence of the disease in the general population of pregnant women, the lack of symptoms in most infected women and the disregard of these problems from health personnel in charge of monitoring pregnancy. Secondary prevention firstly consists in identifying infected women (with history of exposure and positive serology for Chagas disease) and secondly to look for the parasite in newborns from infected mothers. No primary prevention is indeed possible during pregnancy, since the only two drugs are toxic and possibly teratogenic. However, after birth, treatment could be offered to all infected women in order to prevent late complications of the disease and to make an attempt at breaking the chain of MTCT in future pregnancies. |
| PMID: 20131424 [PubMed - indexed for MEDLINE] | |
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| 8. | Bull Soc Pathol Exot. 2009 Dec;102(5):295-9.[Experience of targeted screening of Chagas disease in Ile-de-France][Article in French] Lescure FX, Paris L, Elghouzzi MH, Le Loup G, Develoux M, Touafek F, Mazier D, Pialoux G.Service de maladies infectieuses et tropicales, AP-HP, hôpital Tenon, 4, rue de la Chine, F-75020 Paris, France. xavier.lescure@tnn.aphp.fr Abstract2009 is marked by the centenary of the discovery by Carlos Chagas of Human American Trypanosomiasis. As a result of international cooperation its incidence has been falling in endemic areas, whereas North America and Europe are witnessing an increase in the number of imported cases. In metropolitan France, 18 such cases were reported between 2004 and 2007. Recently, estimates based on immigration figures have been made and suggest that about 1,500 imported cases can be expected in France. The object of this article is to assess the value of targeted screening of an at-risk population, originally from Latin America and now living in the Ile-de-France (area centred on Paris). The serological techniques employed were indirect immunofluorescence (IIF) and, depending on the case, 2 or 3 Elisa tests (Biomérieux, Biokit and Wiener). Trypanosoma cruzi serology was considered positive when the IIF was superior or equal to 200, or when two Elisa's were > 1, or when the IIF was superior or equal to 100 with at least one Elisa > 1. PCR was performed in 48 cases, which were considered to be positive. The tests were carried out on a voluntary basis after a publicity campaign within the Latin American community in the Ile-de-France. In this article, we present the findings of the first year of screening. Two hundred and fifty-four individuals were screened for Chagas' disease between June 2008 and June 2009. The median age was 33 years [11-63], the male/female ratio 102/152. Overall prevalence of positive serology was 23.6% (60/254). For six patients, the results were classified as "uncertain" (discordant serological tests). Of the seropositive group, 87.4% were Bolivian and 100% presented as a chronic form. Of these, 23.6% presented with functional cardiac manifestations and 22% with gastro-intestinal problems. The PCR was positive in 61% of the seropositive individuals. Clinical evaluation together with other investigations and therapeutic intervention is being carried out at present. These results confirm that metropolitan France is subject to the emergence of Chagas' disease in a non-endemic zone. This confirms the value of screening in at-risk populations, in particular because of the recent broadening of indications for antiparasitic treatment. In addition it is relevant to the prevention of vertical transmission or infection via organ donation, which could arise in France. These results also demonstrate continuing difficulties in the interpretation of serological results and the usefulness of PCR, which might increase sensitivity substantially. |
| PMID: 20131423 [PubMed - indexed for MEDLINE] | |
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| 9. | Bull Soc Pathol Exot. 2009 Dec;102(5):291-4.[Chagas disease screening in the blood donor population][Article in French] Assal A, Pelletier B, David B, Tiberghien P.Etablissement français du sang, 20, avenue du Stade-de-France, F-93218 La-Plaine Saint-Denis cedex, France. azzedine.assal@efs.sante.fr AbstractIn May 2007, the French Blood Service (Etablissement français du sang, EFS) introduced systematic screening of at-risk blood donors for anti-Trypanosoma cruzi antibodies. This concerned donors originating from an endemic area, donors with mothers originating from such an area and individuals who had lived in or travelled to endemic areas, whatever the length of their stay. Five samples out of 163,740 were positive, all from individuals originating from an endemic area. One thousand three hundred seventy-four blood donations were considered as equivocal because they had discordant results on the two Elisa tests used in screening. The authors discuss difficulties presented by routine screening of travellers and residents as well as the advantages and drawbacks of the strategy used. They present arguments in favour of its simplification. |
| PMID: 20131422 [PubMed - indexed for MEDLINE] | |
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| 10. | Bull Soc Pathol Exot. 2009 Dec;102(5):280-4.[Sword of Damocles or Russian roulette ... what everyone knows or ought to know about Chagas disease][Article in French] Pays JF.Université del Nordeste, Argentine. pays@necker.fr AbstractIn 2006 the Pan American Health Organisation (PAHO) roughly estimated the prevalence of Chagas disease or American Human Trypanosomiasis, due to Trypanosoma cruzi, still to be eight million cases. The migration of people from the country into towns has resulted, in recent decades, in the urbanisation of this rural disease. Up to the mid-20th century, the epidemiology of the disease was closely linked to the extreme poverty of the peasant population and to their housing, the rancho, which offers a suitable habitat for the vectors and encourages their proliferation. A further barrier has recently been crossed with the arrival in non-endemic areas of numerous seropositive individuals. We shall draw attention in this article to the main clinical signs and to the manner of progression of the disease as well as to the problems posed by treatment of the different phases of this unique condition. It is not fanciful to describe it as resembling an adventure story, because of the place and manner of its discovery and of how the disease unfolds. |
| PMID: 20131420 [PubMed - indexed for MEDLINE] | |
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