What's new for 'Trypanosomatids' in PubMed

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Sent on Thursday, 2010 Aug 19
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

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1.	Mol Pharm. 2010 Aug 17. [Epub ahead of print] The Antitumoral Depsipeptide IB-01212 Kills Leishmania through an Apoptosis-like Process Involving Intracellular Targets. Luque-Ortega JR, Cruz LJ, Albericio F, Rivas L. Centro de Investigaciones Biologicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain, Department of Tumor Immunology, NCMLS 278, Radboud University Nijmegen Medical Centre, Geert Grooteplein 26/28, 6525 GA Nijmegen, The Netherlands, Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, 08028-Barcelona, Spain, CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Barcelona Science Park, Baldiri Reixac 10, 08028-Barcelona, Spain, and Department of Organic Chemistry, University of Barcelona, 08028-Barcelona, Spain. Abstract IB-01212, an antitumoral cyclodepsipeptide isolated from the mycelium of the marine fungus Clonostachys sp., showed leishmanicidal activity at a low micromolar range of concentrations on promastigote and amastigote forms of the parasite. Despite its cationic and amphipathic character, shared with other membrane active antibiotic peptides, IB-01212 did not cause plasma membrane lesions large enough to allow the entrance of the vital dye SYTOX green (MW = 600), even at concentrations causing full lethality of the parasite. Having ruled out massive disruption of the plasma membrane, we surmised the involvement of intracellular targets. Proof of concept for this assumption was provided by the mitochondrial dysfunction caused by IB-01212, which finally caused the death of the parasite through an apoptotic-like process. The size of the cycle, the preservation of the C2 symmetry, and the nature of the bonds linking the two tetrapeptide halves participate in the modulation of the leishmanicidal activity exerted by this compound. Here we discuss the potential of IB-01212 as a lead for new generations of surrogates to be used in chemotherapy treatments against Leishmania .
	PMID: 20715776 [PubMed - as supplied by publisher]

2.	Acta Cytol. 2010 Jul-Aug;54(4):539-45. Comparison of cytologic giemsa and real-time polymerase chain reaction technique for the diagnosis of cutaneous leishmaniasis on scraping smears. Meymandi SS, Bahmanyar M, Dabiri S, Aflatonian MR, Bahmanyar S, Meymandi MS. Kerman Leishmaniasis Research Center, Department of Dermatology, Afzalipour Medical School, Kerman, Iran. Abstract OBJECTIVE: To compare the results of real-time polymerase chain reaction (RT-PCR) for detection of Leishmania DNA in Giemsa-stained skin scraping slides with direct microscopic evaluation of Giemsa-stained skin scrapings and to estimate the sensitivity and specificity of RT-PCR. STUDY DESIGN: We used 30 samples from cases diagnosed with cutaneous leishmaniasis (CL), 16 from clinically suspected individuals but negative in direct microscopic evaluation and 50 normal individuals from nonendemic dry type CL areas. RESULTS: All samples of CL positive and 8 of suspected cases were positive for RT-PCR, and all nonleishmaniasis cases were negative. The sensitivity and specificity of RT-PCR were 100% (95% CI 88-100%) and 88% (95% CI 78-95%), respectively. We also found an inverse association between the number of lympnocytes (OR = 0.90, 95% CI 0.83-0.97%), neutrophils and Leishman bodies.
	PMID: 20715653 [PubMed - in process]