This message contains My NCBI what's new results from the National Center for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM).
Do not reply directly to this message.
Sender's message:
Sent on Thursday, 2011 Mar 24Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
Click here to view complete results in PubMed. (Results may change over time.)
To unsubscribe from these e-mail updates click here.
| PubMed Results |
| 1. | Iran J Immunol. 2011 Mar;8(1):45-51.Immunotherapy with Imiquimod Increases the Efficacy of Glucantime Therapy of Leishmania major Infection.Khalili G, Dobakhti F, Mahmoudzadeh-Niknam H, Khaze V, Partovi F.School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran, e-mail: fdobakhti@Zums.ac.ir. AbstractBackground: Leishmaniasis is a complex disease which presents as visceral, cutaneous and mucocutaneous forms. The current treatment options for this infection are very limited and the immunological state of the host appears to play an important role in the efficacy of the treatment. Immunostimulation through immune response activating agents such as Imiquimod is another rational approach for this purpose. Objective: We assessed the efficacy of immunotherapy with Imiquimod alone or combined with Glucantime for treatment of Leishmania major infection in BALB/c mice. Methods: Treatment efficacy was monitored by determination of thickness and parasite load of infected footpad of mice. Results: The footpad thickness revealed that treatment with Imiquimod plus Glucantime has the highest efficacy. The results were confirmed by parasite load of infected footpad. Our data shows that treatment of Leishmania major infection in BALB/c mice by the combined Imiquimod and Glucantime is more efficient than each drug alone. Conclusion: The combination of Imiquimod with chemotherapy may offer a way for more efficient treatment of leishmaniasis. |
| PMID: 21427495 [PubMed - in process] | |
| 2. | Future Med Chem. 2010 Apr;2(4):539-42.Protease inhibitors as prophylaxis against leishmaniasis: new hope from the major surface protease gp63.Olivier M, Hassani K.McGill University, Department of Microbiology and Immunology, 3775 University Street (Room 610), Montréal, Québec Province, H3A 2B4, Canada. martin.olivier@mcgill.ca. |
| PMID: 21426002 [PubMed - in process] | |
| 3. | ACS Chem Biol. 2011 Mar 22. [Epub ahead of print]Probing Elongating and Branching β-D-Galactosyltransferase Activities in Leishmania Parasites by Making Use of Synthetic Phosphoglycans.Sizova O, Ross A, Ivanova I, Borodkin V, Ferguson MA, Nikolaev A.AbstractProtozoan parasites of the genus Leishmania synthesise lipophosphoglycans (LPGs), phosphoglycans and proteophosphoglycans that contain phosphosaccharide repeat units of [-6)Gal(β1-4)Man(α1-OPO3H-]. The repeat structures are assembled by sequential addition of Manα1-OPO3H and β-Gal. In this study, an UDP-Gal-dependent activity was detected in L. donovani and L. major membranes using synthetic phospho-oligosaccharide fragments of lipophosphoglycan as acceptor substrates. Incubation of a microsomal preparation from L. donovani or L. major parasites with synthetic substrates and UDP-[6-3H]Gal resulted in incorporation of radiolabel into these exogenous acceptors. The [3H]galactose-labeled products were characterised by degradation into radioactive, low molecular mass fragments upon hydrolysis with mild acid and treatment with β-galactosidases. We showed that the activity detected with L. donovani membranes is the elongating β-D-galactosyltransferase associated with LPG phosphosaccharide backbone biosynthesis (eGalT). The eGalT activity showed a requirement for the presence of at least one phosphodiester group in the substrate and it enhanced dramatically when two or three phosphodiester groups were present. Using the same substrates we detected two types of galactosyltransferase activity in L. major membranes: the elongating β-D-galactosyltransferase and a branching β-D-galactosyltransferase (bGalT). Both L. major enzymes required minimum one phosphodiester group present in the substrate, but acceptors with two or three phosphodiester groups were found superior. |
| PMID: 21425873 [PubMed - as supplied by publisher] | |
| 4. | Nat Prod Commun. 2011 Feb;6(2):281-6.Comparative chemical, cytotoxicity and antileishmanial properties of essential oils from Chenopodium ambrosioides.Monzote L, Nance MR, García M, Scull R, Setzer WN.Institute of Medicine Tropical "Pedro Kouri", Havana City, Cuba. monzote@ipk.sld.cu AbstractIn countries where leishmaniasis is endemic, there are not very many treatment alternatives and most options have problems associated with their use. Plants and their natural products constitute good sources of interesting lead compounds that could be potentially active against Leishmania. Chenopodium ambrosioides is a plant that is widely used in popular medicine and its antiparasitic effects have been documented, including the antileishmanial potentialities of Chenopodium oil. The objective of this study was to determine the chemical composition, in-vitro cytotoxicity and antileishmanial activity of essential oils extracted from C. ambrosioides, which received different treatments prior to extraction. The chemical characterization by GC-MS of the three essential oil samples showed similar composition and the major components were alpha-terpinene (17.0-20.7%), p-cymene (20.2-21.1%) and ascaridole (30.5-47.1%). The essential oils exhibited similar antileishmanial activities against intracellular amastigote form, with IC50 values between 4.7 and 12.4 microg/mL. However, a lower cytotoxicity was displayed by the essential oil extracted from fresh green vegetable material, which was statistically different (P < 0.05) from the other samples. This study demonstrated that the prior treatment of plant material did not interfere with the antiparasitic activity of essential oils from C. ambrosioides but did change their cytotoxicity, which should be taken into account in further studies. |
| PMID: 21425695 [PubMed - in process] | |
| 5. | Nat Prod Commun. 2011 Feb;6(2):231-4.Leishmanicidal and cytotoxic activities of extracts and naturally-occurring compounds from two Lauraceae species.Sánchez-Suárez J, Coy-Barrera E, Cuca LE, Delgado G.Grupo de Investigación en Inmunotoxicología, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, 57+1+3165120 Bogotá, Colombia. AbstractThe in vitro leishmanicidal effects of ethanolic extracts and fifteen naturally-occurring compounds (five lignans, eight neolignans, a diterpene and a dihydrochalcone), obtained from Pleurothyrium cinereum and Ocotea macrophylla, were evaluated on promastigotes of Leishmania panamensis and L. braziliensis. In addition, in order to determine the selective action on Leishmania species as a safety principle, in vitro cytotoxicity on J774 cells was also evaluated for test compounds and extracts. One extract and seven compounds showed activity against Leishmania parasites at different levels. Dihydroflavokawin B (8) was found to be the most potent antileishmanial compound on both parasites, whilst (+)-otobaphenol (14), was found to be the most selective compound on L. panamensis. |
| PMID: 21425681 [PubMed - in process] | |
| 6. | Vet Parasitol. 2010 Dec 15;174(3-4):323-7. Epub 2010 Sep 15.The role of wild rodents in the transmission of Trypanosoma evansi infection in an endemic area of the Canary Islands (Spain).Rodríguez NF, Tejedor-Junco MT, Hernández-Trujillo Y, González M, Gutiérrez C.Department of Animal Medicine and Surgery, Veterinary Faculty, University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Canary Islands, Spain. AbstractTrypanosoma evansi was diagnosed for the first time in camels in the Canary Islands in 1997. Several sanitary measures including treatment of infected animals were taken; however, nowadays a little area is still infected. In order to determine possible reservoirs 138 wild rodents were trapped, 64 of them in the infected farms and the remaining 74 in other areas. The captured species were Rattus rattus (24), Rattus norvegicus (69) and Mus musculus domesticus (45). Serological (CATT/T. evansi), parasitological (micro-Hematocrit Centrifugation technique and stained smears) and molecular (PCR) methods for T. evansi and T. lewisi were used as diagnostic methods. None of the examined rodents was positive for T. evansi; 18, however, showed motile trypanosomes at micro-Hematocrit Centrifugation technique and resulted positive for T. lewisi by PCR. The results would suggest that the studied rodent species would not play a relevant role in the epidemiology of T. evansi infection in Canaries. Copyright © 2010 Elsevier B.V. All rights reserved. |
| PMID: 20888126 [PubMed - indexed for MEDLINE] | |
| Related citations | |
 |
No comments:
Post a Comment