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Sent on Saturday, 2011 Mar 26Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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| PubMed Results |
| 1. | Anal Bioanal Chem. 2011 Mar 25. [Epub ahead of print]Using multidimensional projection techniques for reaching a high distinguishing ability in biosensing.Paulovich FV, Maki RM, de Oliveira MC, Colhone MC, Santos FR, Migliaccio V, Ciancaglini P, Perez KR, Stabeli RG, Perinoto AC, Oliveira ON Jr, Zucolotto V.Instituto de Ciências Matemáticas e de Computação, USP, CP 668, São Carlos, 13560-970, São Paulo, Brazil. AbstractRecent advances in the control of molecular engineering architectures have allowed unprecedented ability of molecular recognition in biosensing, with a promising impact for clinical diagnosis and environment control. The availability of large amounts of data from electrical, optical, or electrochemical measurements requires, however, sophisticated data treatment in order to optimize sensing performance. In this study, we show how an information visualization system based on projections, referred to as Projection Explorer (PEx), can be used to achieve high performance for biosensors made with nanostructured films containing immobilized antigens. As a proof of concept, various visualizations were obtained with impedance spectroscopy data from an array of sensors whose electrical response could be specific toward a given antibody (analyte) owing to molecular recognition processes. In addition to discussing the distinct methods for projection and normalization of the data, we demonstrate that an excellent distinction can be made between real samples tested positive for Chagas disease and Leishmaniasis, which could not be achieved with conventional statistical methods. Such high performance probably arose from the possibility of treating the data in the whole frequency range. Through a systematic analysis, it was inferred that Sammon's mapping with standardization to normalize the data gives the best results, where distinction could be made of blood serum samples containing 10(-7) mg/mL of the antibody. The method inherent in PEx and the procedures for analyzing the impedance data are entirely generic and can be extended to optimize any type of sensor or biosensor. |
| PMID: 21437775 [PubMed - as supplied by publisher] | |
| 2. | An Bras Dermatol. 2011 Feb;86(1):55-63.[American cutaneous leishmaniasis: clinical, epidemiological and laboratory studies conducted at a university teaching hospital in Campo Grande, Mato Grosso do Sul, Brazil.][Article in Portuguese] Murback ND, Hans Filho G, Nascimento RA, Nakazato KR, Dorval ME.Faculdade de Medicina Dr. Hélio Mandetta, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. AbstractBACKGROUND: American cutaneous leishmaniasis is a disease with a wide variety of clinical manifestations that is expanding throughout Brazil, the state of Mato Grosso do Sul constituting a significant endemic area. OBJECTIVES: To evaluate the clinical, epidemiological and laboratory characteristics of patients with American cutaneous leishmaniasis. Patients were recruited among those attending the Maria Aparecida Pedrossian Teaching Hospital of the Federal University of Mato Grosso do Sul, Brazil. METHODS: This was a cross-sectional, observational study conducted using a descriptive and analytical approach. Data from patients suspected of having American cutaneous leishmaniasis who were receiving care at this institute between 1998 and 2008 and were referred to the institute's parasitology laboratory for confirmation of diagnosis were evaluated retrospectively. Clinical and laboratory criteria were taken into consideration for the inclusion of patients to the study. RESULTS: Forty-seven patients were included in the study, the majority of whom were male and between 45 and 59 years of age. Most had the cutaneous form of the disease with a single, ulcerated lesion on exposed areas of the body, which had generally been present for periods of less than six months. Mucosal involvement increased with age and was highest in patients who had sought medical care at a later stage. The Montenegro skin test showed the highest sensitivity. Finding the parasite was more difficult in older lesions. CONCLUSION: Suspicion of the disease at an early stage is of extreme importance for a precise diagnosis. A combination of parasitological and immunological tests renders laboratory diagnosis more reliable. |
| PMID: 21437523 [PubMed - as supplied by publisher] | |
| 3. | Transplantation. 2011 Apr 15;91(7):806-808.Visceral Leishmaniasis in Liver Transplant Recipients From an Endemic Area.Clemente WT, Faria LC, Romanelli RM, Lima SS, Cortes JR, Oliveira AP, Carvalho AL, Ferreira AR, Lima AS.1 Infection Control Committee; Hospital das Clínicas/UFMG (Clemente, Stella Lima) 2 Study and Research Group on Health; Care and Hospital Epidemiology-; Associated Infections (GREPI) (Clemente, Romanelli, Stella Lima, Cortes, Oliveira) 3 Laboratory Medicine Department; Medical School UFMG (Clemente) 4 Alpha Institute of Gastroenterology - Liver Transplant Unit; Hospital das Clínicas/UFMG (Clemente, Faria, Ferreira, Agnaldo Lima) 5 Department of Internal Medicine; Medical School UFMG (Faria) 6 Department of Pediatrics; Medical School UFMG (Romanelli, Carvalho, Ferreira) 7 Orestes Diniz Training and Referral; Infectious Diseases Center (Carvalho) 8 Department of Surgery; Medical School UFMG; UFMG - Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (Agnaldo Lima). |
| PMID: 21436655 [PubMed - as supplied by publisher] | |
| 4. | Mol Pharmacol. 2011 Mar 24. [Epub ahead of print]The diamidine diminazene aceturate is a substrate for the High Affinity Pentamidine Transporter: implications for the development of high resistance levels in trypanosomes.Teka IA, Kazibwe AJ, El-Sabbagh N, Al-Salabi MI, Ward CP, Eze AA, Munday JC, Maeser P, Matovu E, Barrett MP, De Koning HP.1 University of Glasgow, UK; AbstractAfrican trypanosomiasis is a disease of humans and livestock in many areas south of the Sahara. Resistance to the few existing drugs is a major impediment to control of these diseases and we here investigate how resistance to the main veterinary drug diminazene aceturate correlates with changes in drug transport in resistant strains. The strain tbat1(-/-), lacking the TbAT1/P2 aminopurine transporter previously implicated in diminazene transport, was adapted to higher levels of diminazene resistance. The resulting cell line was designated ABR and was highly cross-resistant to other diamidines, and moderately resistant to cymelarsan. Procyclic trypanosomes were shown to be a convenient model to study diamidine uptake in T. b. brucei given (1) the lack of TbAT1/P2 and (2) a ten-fold higher activity of the High Affinity Pentamidine Transporter HAPT1. Diminazene could be transported by HAPT in procyclic trypanosomes; this drug transport activity was lacking in the ABR line, as previously reported for the pentamidine-adapted line B48. The K(m) for diminazene transport in bloodstream tbat1(-/-) trypanosomes was consistent with uptake by HAPT1. Diminazene transport in ABR and B48 cells was reduced compared to tbat1(-/-) but their resistance phenotype was different: B48 displayed higher levels of resistance to pentamidine and the melaminophenyl arsenicals, whereas ABR displayed higher resistance to diminazene. These results establish loss of HAPT1 function as a contributing factor to diminazene resistance but equally demonstrate for the first time that adaptations other than those determining initial rates of drug uptake contribute to diamidine and arsenical resistance in African trypanosomes. |
| PMID: 21436312 [PubMed - as supplied by publisher] | |
| 5. | Bioorg Med Chem. 2011 Mar 4. [Epub ahead of print]Anti-tumor and anti-leishmanial evaluations of 1,3,4-oxadiazine, pyran derivatives derived from cross-coupling reactions of β-bromo-6H-1,3,4-oxadiazine derivatives.Mohareb RM, Schatz J.Faculty of Pharmacy, Organic Chemistry Department, October University for Modern Science and Arts University, El-Wahaat Road, October City, Egypt; Department of Chemistry, Faculty of Science, Cairo University, Giza, A.R., Egypt. AbstractCyanoacetylhydrazine reacted with the ω-bromoacetophenones 2a,b to give hydrazide-hydrazone derivatives 3a,b. The latter products were cyclized to the 1,3,4-oxadiazine derivatives 4a,b. Bromination of the latter products gave the 6-bromo-6H-1,3,4-oxadiazine derivatives 5a,b which underwent a series of cross-coupling reactions. The antitumor evaluation of the newly synthesized products against the three cancer cells namely breast adenocarcinoma (MCF-7), non-small cell lung cancer (NCI-H460) and CNS cancer (SF-268) showed that some of them have high inhibitory effect towards three cell lines which is higher than the standard. Moreover, the anti-leishmanial activity of the newly synthesized product was tested on Leishmania donovani amastigotes showed that some compounds have high activity. Copyright © 2011. Published by Elsevier Ltd. |
| PMID: 21435889 [PubMed - as supplied by publisher] | |
| 6. | Nature. 2011 Mar 10;471(7337):173-4.Host-pathogen interaction: Culprit within a culprit.Olivier M. |
| PMID: 21390122 [PubMed - indexed for MEDLINE] | |
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