What's new for 'Trypanosomatids' in PubMed

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Sent on Tuesday, 2012 March 13
Search: kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"

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PubMed Results

Items 1 - 5 of 5

1.	J Biol Chem. 2012 Mar 9. [Epub ahead of print] The Protein Translocase of Mitochondrial Inner Membrane in Trypanosoma brucei. Singha UK, Hamilton V, Duncan MR, Weems E, Tripathi MK, Chaudhuri M. Source Meharry Medical College, United States; Abstract Translocases of mitochondrial inner membrane (TIMs) are multi-protein complexes. The only Tim component so far characterized in kinetoplastid parasites such as Trypanosoma brucei is Tim17 (TbTim17), which is essential for cell survival and for mitochondrial protein import. Here, we report that TbTim17 is present in a protein complex of about 1,100 kDa, which is much larger than the TIM complexes found in fungi and mammals. Depletion of TbTim17 in T. brucei impairs the mitochondrial import of cytochrome oxidase subunit IV (COIV), an N-terminal signal-containing protein. Pretreatment of isolated mitoplast with the anti-TbTim17 antibody inhibited import of COIV, indicating a direct involvement of the TbTim17 in the import process. Purification of the TbTim17-containing protein complex from the mitochondrial membrane of T. brucei by tandem affinity chromatography revealed that TbTim17 associates with seven unique as well as a few known T. brucei mitochondrial proteins. Depletion of three of these novel proteins, i.e., TbTim47, TbTim54, and TbTim62 significantly decreased mitochondrial protein import in vitro. In vivo targeting of a newly synthesized mitochondrial matrix protein MRP2 was also inhibited due to depletion of TbTim17, TbTim54 and TbTim62. Co-precipitation analysis confirmed the interaction of TbTim54 and TbTim62 with TbTim17 in vivo. Overall, our data reveal that TbTim17, the single homolog of Tim17/22/23 family proteins, is present in a unique TIM complex consisting of novel proteins in T. brucei and is critical for mitochondrial protein import.
	PMID: 22408251 [PubMed - as supplied by publisher]

2.	Exp Parasitol. 2012 Mar 3. [Epub ahead of print] Terminal galactosylation of glycoconjugates in Plasmodium falciparum asexual blood stages and Trypanosoma brucei bloodstream trypomastigotes. Ramasamy R, Field MC. Source Department of Pathology, University of Cambridge, Cambridge, UK; Institute of Health Sciences, Universiti Brunei Darussalam, Gadong, Brunei Darussalam. Abstract There is definitive biochemical evidence for the presence of terminal α-galactosyl residues (α-gal) in the N-linked oligosaccharides and glycophosphatidylinositol anchors (GPI anchors) of the variant surface glycoprotein of Trypanosoma brucei bloodstream trypomastigotes. Indirect evidence also exists for α-gal in Plasmodium falciparum asexual blood stage glycoproteins and glycolipids. The occurrence of α-gal in glycoproteins and glycolipids of T. brucei bloodstream trypomastigotes and P. falciparum late asexual blood stages was investigated by the binding of α-gal-specific Bandeirea simplicifolia B4 lectin 1 (BSB4), incorporation of [(3)H]galactose from UDP-[(3)H]galactose into glycoproteins and glycolipids in microsomes in vitro, and bioinformatic searches for galactosyl-transferase coding sequences. The findings confirm the presence of α-gal in a spectrum of T. brucei bloodstream trypomastigote glycoproteins and glycolipids and indicate its relative absence from P. falciparum asexual blood stage glycoconjugates. Copyright © 2012 Elsevier Inc. All rights reserved.
	PMID: 22406352 [PubMed - as supplied by publisher]

3.	Mol Biochem Parasitol. 2012 Mar 3. [Epub ahead of print] Trypanosoma brucei solanesyl-diphosphate synthase localizes to the mitochondrion. Lai DH, Bontempi EJ, Lukeš J. Source Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK. Abstract Polyprenyl-diphosphate synthase is a key enzyme in the biosynthesis of ubiquinone, a molecule considered essential for a typical eukaryotic cell. Its orthologue in the American stercorarian flagellate Trypanosoma cruzi, solanesyl diphosphate synthase, has been previously localized into the glycosomes. We wondered whether this unique cellular localization is shared by other trypanosome species. Using digitonin permeabilization, immunofluorescence and in situ tagging techniques, we show that in Trypanosoma brucei, the African salivarian flagellate, the enzyme localizes to the mitochondrion. Copyright © 2012. Published by Elsevier B.V.
	PMID: 22406034 [PubMed - as supplied by publisher]

4.	Travel Med Infect Dis. 2012 Mar 7. [Epub ahead of print] Imported cutaneous leishmaniasis in a short-term traveler returning from Central Mali - The role of PCR. Kelly P, Baudry T, Peyron F. Source Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA. Abstract Leishmaniasis is a parasitic infection caused by the obligate intracellular protazoa leishmania. The most commonly encountered form is cutaneous leishmaniasis (CL), which generally manifests as a chronic, painless ulcer. Recent increases in the incidence of CL worldwide due in large part to increased immigration and international travel, combined often with the lack of familiarity with the disease in non-endemic settings, pose the continued problems of delayed diagnosis and inappropriate treatment. A case is described of imported cutaneous leishmaniasis occurring in a 48 year-old male who presented with multiple painless, progressively ulcerating lesions after returning from a one week trip to Bandiagara, Mali, West Africa. After four months of misdiagnoses and ineffective treatments, he was referred to a tropical disease specialist where the diagnosis was made with a skin biopsy followed by a tissue impression smear, culture and PCR. Appropriate treatment was initiated and the lesions resolved with minimal scarring. The goals of this case report are threefold: first, to stress the importance of associating chronic ulcers in a traveler with potential cutaneous leishmaniasis; second, to emphasize the clinical utility of PCR for the diagnosis; and third, to discuss the clinical approach to treatment. Copyright © 2012 Elsevier Ltd. All rights reserved.
	PMID: 22405679 [PubMed - as supplied by publisher]

5.	Enferm Infecc Microbiol Clin. 2012 Mar 7. [Epub ahead of print] [Visceral leishmaniasis diagnosed from a colon biopsy.] [Article in Spanish] Jimeno A, Morales E, Peñalver E, Ladrón de Guevara S. Source Sección de Enfermedades Infecciosas, Servicio de Medicina Interna, Hospital Santa Lucía, Cartagena, Murcia, España.
	PMID: 22405140 [PubMed - as supplied by publisher]