What's new for 'Trypanosomatids' in PubMed

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Sent on Wednesday, 2011 Feb 09
Search kinetoplastids OR kinetoplastid OR Kinetoplastida OR "trypanosoma brucei" OR leishmania OR brucei OR leishmaniasis OR "African trypanosomiasis"
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PubMed Results

Items 1 - 4 of 4

1.	Biochimie. 2011 Feb 4. [Epub ahead of print] Cell cycle arrest evidence, parasiticidal and bactericidal properties induced by L-amino acid oxidase from Bothrops atrox snake venom. Alves Paiva RD, de Freitas Figueiredo R, Antonucci GA, Paiva HH, Pires Bianchi MD, Rodrigues KC, Lucarini R, Caetano RC, Pietro RC, Gomes Martins CH, de Albuquerque S, Sampaio SV. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Abstract The present article describes an L-amino acid oxidase from Bothrops atrox snake venom as with antiprotozoal activities in Trypanosoma cruzi and in different species of Leishmania (Leishmania braziliensis, Leishmania donovani and Leishmania major). Leishmanicidal effects were inhibited by catalase, suggesting that they are mediated by H(2)O(2) production. Leishmania spp cause a spectrum of diseases, ranging from self-healing ulcers to disseminated and often fatal infections, depending on the species involved and the host's immune response. BatroxLAAO also displays bactericidal activity against both Gram-positive and Gram-negative bacteria. The apoptosis induced by BatroxLAAO on HL-60 cell lines and PBMC cells was determined by morphological cell evaluation using a mix of fluorescent dyes. As revealed by flow cytometry analysis, suppression of cell proliferation with BatroxLAAO was accompanied by the significant accumulation of cells in the G0/G1 phase boundary in HL-60 cells. BatroxLAAO at 25 μg/mL and 50 μg/mL blocked G0-G1 transition, resulting in G0/G1 phase cell cycle arrest, thereby delaying the progression of cells through S and G2/M phase in HL-60 cells. This was shown by an accentuated decrease in the proportion of cells in S phase, and the almost absence of G2/M phase cell population. BatroxLAAO is an interesting enzyme that provides a better understanding of the ophidian envenomation mechanism, and has biotechnological potential as a model for therapeutic agents. Copyright © 2011 Elsevier Masson SAS. All rights reserved.
	PMID: 21300133 [PubMed - as supplied by publisher]
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2.	Br J Dermatol. 2011 Feb 7. doi: 10.1111/j.1365-2133.2011.10240.x. [Epub ahead of print] Influence of Notch System In The Therapeutic Response Of American Tegumentary Leishmaniasis. Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RD, Pirmez C. Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro - RJ Instituto de Pesquisa Evandro Chagas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro - RJ Serviço de Otorrinolaringologia da Santa Casa de Misericórdia de São Paulo. Abstract Background:  American Tegumentary Leishmaniasis (ATL) evolution and therapeutic outcome depend upon many factors, including the balance between Th1 and Th2 cytokines to control parasite multiplication and the lesion extension. Other cytokines known for their role in inflammatory processes such as IL-17 or IL-18 as well as factors controlling keratinocyte differentiation and the inflammatory process in the skin, like Notch system, could also be involved in the disease outcome. Notch receptors are a group of transmembrane proteins that regulate cell fate decisions during development and adulthood in many tissues, including keratinocyte differentiation and T-cell lineage commitment, depending on its activation by specific groups of ligands (Delta-like or Jagged). Objective:  To compare the in situ expression of Notch system proteins (receptors, ligands and transcriptional factors) and cytokines possibly involved in the disease outcome (IL-17, IL-18, IL-23 and TGFβ) in ATL cutaneous and mucosal lesions, according to the therapeutic response with N-metil glucamine. Methods:  Cutaneous and mucosal biopsies obtained from patients prior to therapy with N-metil glucamine were analysed by immunohistochemistry and real-time PCR. Results:  Notch receptors and Delta-like ligands were found increased in patients with ATL, particularly those with poor response to therapy or mucosal lesions. Conclusion:  The rise of Notch receptors and Delta-like ligands in patients with a poor response to treatment suggests that these patients would require a more aggressive therapeutic approach or at least a more thorough and rigorous follow-up. Copyright © 2011 British Association of Dermatologists.
	PMID: 21299543 [PubMed - as supplied by publisher]
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3.	Cell Mol Biol (Noisy-le-grand). 2010 Jun 15;56 Suppl:OL1318-23. The trypanocidal activity of the alkaloid oliverine involves inhibition of DNA synthesis. Garro HA, Juri Ayub M, Nieto M, Lucero Estrada C, Pungitore CR, Tonn CE. INTEQUI-CONICET, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis (UNSL), Chacabuco y Pedernera, San Luis, Argentina. Abstract The Trypanosoma cruzi parasite is an etiologic agent of the American trypanosomiasis called Chagas disease. This pathology affects more than 24 million persons and represents one of the most important public health problems in Latin America. Taking into account this, it is necessary the search of new antitrypanosomal agents that show a major level of efficacy and minor indexes of toxicity in affected patients. Vast source of them are the natural products from plants with enormous structural diversity. A particular type of these compounds is represented by aporphinoid alkaloids. In our experiments, anonaine (2), oliverine (3) and guatterine (5) displayed antitrypanosomal activity. The compound 3 showed the most important activity with an IC50 = 12.00 ± 0.36 μM. Its mechanism of action may include inhibition of DNA synthesis.
	PMID: 20937218 [PubMed - indexed for MEDLINE]
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4.	Ann Diagn Pathol. 2010 Oct;14(5):337-40. Chagas disease and gynecologic neoplasias. Dominical VM, Cavellani CL, Rocha LP, Corrêa RR, Pereira Gde A, Teixeira Vde P. Triangulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil. Abstract The inflammation caused by Trypanosoma cruzi produces irritation and cell proliferation and may contribute to the development of cancer. The objective was to determine the occurrence of gynecologic neoplasia (GN) and demographic characteristics in patients with Chagas disease (CD). We used protocols of 671 autopsies between 1976 and 2008. The patients were divided into 3 groups: with GN and CD, only with CD, and only with GN. The 2 diseases were observed in 4.5% of patients with a mean age of 47.6 years and who were predominantly white. The megaesophagus and megacolon were more frequent in the group with only CD. The most common benign neoplasm was uterine leiomyoma, and malignant, carcinoma of the cervix. We conclude that the epidemiological profile of patients with CD and GN was similar to the other groups, and the CD was found not to be a risk factor or protective against the development of GN. Copyright © 2010 Elsevier Inc. All rights reserved.
	PMID: 20850696 [PubMed - indexed for MEDLINE]
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